Distribution of Chlamydia pneumoniae DNA in Atherosclerotic Carotid Arteries: Significance for Sampling Procedures

Cochrane, Melanie, Pospischil, Andreas, Walker, Philip J., Gibbs, Harry, & Timms, Peter (2003) Distribution of Chlamydia pneumoniae DNA in Atherosclerotic Carotid Arteries: Significance for Sampling Procedures. Journal of Clinical Microbiology, 41(4), pp. 1454-1457.

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Despite extensive efforts to confirm a direct association between Chlamydia pneumoniae and atherosclerosis, different laboratories continue to report a large variability in detection rates. In this study, we analyzed multiple sections from atherosclerotic carotid arteries from 10 endartectomy patients to determine the location of C. pneumoniae DNA and the number of sections of the plaque required for analysis to obtain a 95% confidence of detecting the bacterium. A sensitive nested PCR assay detected C. pneumoniae DNA in all patients at one or more locations within the plaque. On average, 42% (ranging from 5 to 91%) of the sections from any single patient had C. pneumoniae DNA present. A patchy distribution of C. pneumoniae in the atherosclerotic lesions was observed, with no area of the carotid having significantly more C. pneumoniae DNA present. If a single random 30-µm-thick section was tested, there was only a 35.6 to 41.6% (95% confidence interval) chance of detecting C. pneumoniae DNA in a patient with carotid artery disease. A minimum of 15 sections would therefore be required to obtain a 95% chance of detecting all true positives. The low concentration and patchy distribution of C. pneumoniae DNA in atherosclerotic plaque appear to be among the reasons for inconsistency between laboratories in the results reported.

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28 citations in Scopus
25 citations in Web of Science®
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ID Code: 10066
Item Type: Journal Article
Refereed: Yes
Additional Information: Articles free to read on journal website after 6 months
DOI: 10.1128/JCM.41.4.1454-1457.2003
ISSN: 1098-660X
Divisions: Past > QUT Faculties & Divisions > Faculty of Science and Technology
Copyright Owner: Copyright 2003 American Society for Microbiology
Deposited On: 10 Oct 2007 00:00
Last Modified: 05 Feb 2015 00:35

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