Characterisation of the Dunaliella tertiolecta RbcS genes and their promoter activity in Chlamydomonas reinhardtii

Walker, Tara L., Becker, Douglas K., & Collet, Christopher C. (2005) Characterisation of the Dunaliella tertiolecta RbcS genes and their promoter activity in Chlamydomonas reinhardtii. Plant Cell Reports, 23(10-11), pp. 727-735.

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The availability of highly active homologous promoters and terminators is critical in the development of a transformation system for the unicellular microalga Dunaliella tertiolecta. To facilitate transformation of this species, we isolated and characterised two native ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit genes (RbcS) including flanking sequences. The two non-allelic cDNA sequences share approximately 80% identity and have approximately 60% identity to the RbcS genes of Chlamydomonas reinhardtii. The D. tertiolecta RbcS promoter and 3 untranslated regions were shown to drive expression of the bleomycin resistance gene (ble) in C. reinhardtii. This is the first demonstration of a heterologous algal promoter being used to drive transgene expression in C. reinhardtii. In addition, promoter deletions were shown to further increase transformation efficiency.

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19 citations in Scopus
21 citations in Web of Science®
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ID Code: 10165
Item Type: Journal Article
Refereed: Yes
Keywords: Dunaliella tertiolecta, RbcS, Promoter, Chlamydomonas reinhardtii, ble
DOI: 10.1007/s00299-004-0884-x
ISSN: 1432-203X
Divisions: Past > QUT Faculties & Divisions > Faculty of Science and Technology
Past > Schools > School of Life Sciences
Copyright Owner: Copyright 2005 Springer
Copyright Statement: The original publication is available at SpringerLink
Deposited On: 15 Oct 2007 00:00
Last Modified: 17 Mar 2015 01:21

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