Assessment of Stromal Keratocytes and Tear Film Inflammatory Mediators During Extended Wear of Contact Lenses.

Kallinikos, Panagiotis, Morgan, Philip, & Efron, Nathan (2006) Assessment of Stromal Keratocytes and Tear Film Inflammatory Mediators During Extended Wear of Contact Lenses. Cornea : the journal of cornea and external disease, 25(1), pp. 1-10.


Purpose: To monitor quantitative changes in stromal keratocyte density and the level of tear film inflammatory mediators following extended contact lens wear.

Methods: Twenty-two subjects aged 32 +/- 11 years participated in this cross-sectional study. Eleven subjects had worn silicone hydrogel (Si-H) lenses on a 30-day continuous wear basis for 12 months. Eleven subjects had worn rigid gas permeable lenses on the same basis for 12 months. Eleven age-matched control subjects were also recruited. Ultrasound pachometry, confocal microscopy, and tear fluid sample collection were performed on all subjects. Tear samples were assayed for epidermal growth factor (EGF), hepatocyte growth factor (HGF) and interleukin (IL)-8.

Results: Corneal thickness was similar for all subject groups. Total keratocyte density was not different between the 3 groups; however, keratocyte density was lower for rigid lens wearers in the anterior to mid stroma and lower for Si-H lens wearers in the posterior stroma compared with control subjects. Rigid lens wearers exhibited an irregular keratocyte distribution across the corneal stroma. EGF concentration and rate of release was greater in the tears collected from the rigid lens wearers and Si-H lens wearers, and IL-8 concentration was higher in the samples collected from the rigid lens wearers compared with the samples collected from the control subjects.

Conclusions: Mechanical stimulation of the corneal surface due to the physical presence of a contact lens and the consequent release of inflammatory mediators may account for a loss or redistribution of keratocytes.

Impact and interest:

39 citations in Scopus
34 citations in Web of Science®
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ID Code: 10185
Item Type: Journal Article
Refereed: Yes
Additional Information: Self-archiving of the author-version is not yet supported by this publisher.
For more information, please refer to the journal’s website (see hypertext link) or contact the author.
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Keywords: confocal microscope, contact lens wear, keratocyte density, corneal thickness, inflammatory mediators epidermal growth, factor, human corneal keratocyte, in, situ keratomileusis, confocal microscopy, photorefractive keratectomy, lacrimal gland, increased release, density, fluid, vivo
ISSN: 1536-4798
Subjects: Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > OPTOMETRY AND OPHTHALMOLOGY (111300) > Optometry and Ophthalmology not elsewhere classified (111399)
Divisions: Current > Research Centres > Centre for Health Research
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright 2006 Lippincott Williams & Wilkins
Deposited On: 17 Oct 2007 00:00
Last Modified: 10 Aug 2011 16:16

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