Potential pitfalls of radiolabel adsorption to ceramic biomaterials

Parker, Tony J., Upton, Zee, Vellinga, Douwe, Wei, Mei, & Leavesley, David I. (2005) Potential pitfalls of radiolabel adsorption to ceramic biomaterials. Journal of Biomedical Materials Research Part A, 72A(4), 363 -372.

View at publisher


The use of radiolabeled precursor molecules for the metabolic analysis of cell functions is commonplace. Tritiated thymidine, in particular, has been used to quantitate cellular proliferation in numerous cells, including osteoblasts, when cultured on various biomaterials. Our aim was to assess cellular protein synthesis and proliferation, on a range of fluoride ion-substituted hydroxyapatites. Initially, we used a classical metabolic analysis strategy with radiolabeled tracer molecules. Our results suggested that these materials supported enhanced protein synthesis and proliferation of SaOS-2 human osteoblast-like cells. However, control samples also revealed enhanced adsorption of the radiolabeled tracer. We have shown that this arises because partially fluoride ion-substituted hydroxyapatite exhibits enhanced adsorptive characteristics of radiolabeled leucine and thymidine over tissue culture plastic, hydroxyapatite, and fluoroapatite. Moreover, manual cell count data obtained through SEM analysis showed no significant difference in cell proliferation between any of the materials, further indicating that our initial results were artifacts. These results highlight the use and reporting of appropriate cell-free controls are critical in bioassays examining functional responses of cells to biomaterials, and if absent, may confound accurate data interpretation. Our findings have general implications for investigations of cell function on other novel ceramic biomaterials.

Impact and interest:

0 citations in Scopus
Search Google Scholar™

Citation counts are sourced monthly from Scopus and Web of Science® citation databases.

These databases contain citations from different subsets of available publications and different time periods and thus the citation count from each is usually different. Some works are not in either database and no count is displayed. Scopus includes citations from articles published in 1996 onwards, and Web of Science® generally from 1980 onwards.

Citations counts from the Google Scholar™ indexing service can be viewed at the linked Google Scholar™ search.

ID Code: 10256
Item Type: Journal Article
Refereed: Yes
Additional Information: For more information, please refer to the journal's website (see hypertext link) or contact the author.
Keywords: adsorption, biomaterial, ceramic, osteoblast, proliferation
DOI: 10.1002/jbm.a.30247
ISSN: 1552-4965
Divisions: Past > QUT Faculties & Divisions > Faculty of Science and Technology
Copyright Owner: Copyright 2005 John Wiley & Sons
Deposited On: 19 Oct 2007 00:00
Last Modified: 29 Feb 2012 13:13

Export: EndNote | Dublin Core | BibTeX

Repository Staff Only: item control page