The Androgen Receptor Can Promote Beta-Catenin Nuclear Translocation Independently of Adenomatous Polyposis Coli

Mulholland, David, Cheng, Helen, Reid, Kimberly, Rennie, Paul S., & Nelson, Colleen C. (2002) The Androgen Receptor Can Promote Beta-Catenin Nuclear Translocation Independently of Adenomatous Polyposis Coli. Journal of Biological Chemistry, 277(20), pp. 17933-17943.

View at publisher (open access)


We provide evidence that the androgen receptor (AR) can promote nuclear translocation of -catenin in LNCaP and PC3 prostate cancer cells. Using AR-expressing cells (LNCaP) and non-AR-expressing cells (PC3) we showed by time course cell fractionation that the AR can shuttle -catenin into the nucleus when exposed to exogenous androgen. Cells exposed to the synthetic androgen, R1881, show distinct, punctate, nuclear co-localization of the AR and -catenin. We further showed that the AR does not interact with adenomatous polyposis coli or glycogen synthase kinase-3 and, therefore, conclude that androgen-mediated transport of -catenin occurs through a distinct pathway. The minimal necessary components of the AR and -catenin required for binding nuclear accumulation of -catenin nuclear import appears to be the DNA/ligand binding regions and the Armadillo repeats of -catenin. We also employed a novel DNA binding assay to illustrate that -catenin has the capacity to bind to the probasin promoter in an AR-dependent manner. The physiological relevance of AR-mediated transport of -catenin and binding to an AR promoter appeared to be a substantial increase in AR transcriptional reporter activity. AR-mediated import represents a novel mode of nuclear accumulation of -catenin.

Impact and interest:

122 citations in Scopus
Search Google Scholar™
116 citations in Web of Science®

Citation counts are sourced monthly from Scopus and Web of Science® citation databases.

These databases contain citations from different subsets of available publications and different time periods and thus the citation count from each is usually different. Some works are not in either database and no count is displayed. Scopus includes citations from articles published in 1996 onwards, and Web of Science® generally from 1980 onwards.

Citations counts from the Google Scholar™ indexing service can be viewed at the linked Google Scholar™ search.

ID Code: 10269
Item Type: Journal Article
Refereed: Yes
Additional Information: Articles free to read on journal website after 12 months
Additional URLs:
DOI: 10.1074/jbc.M200135200
ISSN: 0021-9258
Divisions: Past > QUT Faculties & Divisions > Faculty of Science and Technology
Copyright Owner: Copyright 2002 American Society for Biochemistry and Molecular Biology
Deposited On: 19 Oct 2007 00:00
Last Modified: 04 Feb 2015 06:05

Export: EndNote | Dublin Core | BibTeX

Repository Staff Only: item control page