Enhanced shoot regeneration in nine Australian wheat cultivars by spermidine and water stress treatments

Khanna, Harjeet Kaur & Daggard, Grant E. (2001) Enhanced shoot regeneration in nine Australian wheat cultivars by spermidine and water stress treatments. Australian Journal of Plant Physiology (Renamed 2002: Functional Plant Biology: an international journal of plant function), 28(12), pp. 1243-1247.

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Abstract

The regeneration potential of ageing calli initiated from isolated scutella of immature embryos was increased in nine elite Australian cultivars (QT7208, QT9685, QT7709, Kennedy, Lang, Sunvale, Giles, Petrie and Veery) of wheat (Triticum aestivum L.). Firstly, the effects of 4–32 h of dehydration stress on regeneration of 4- to 20-week old calli were evaluated. Cultivars such as Veery, Kennedy and Sunvale showed significant improvement in regeneration from calli up to 12-weeks old that had undergone 16 h of dehydration stress. Secondly, 4- to 20-week old callus cultures were treated with 0.05–5 mM spermidine to evaluate its effect on regeneration. While spermidine had a negative effect on regeneration from 4-week old calli at all tested concentrations (as compared with untreated controls), there was a 3–50% improvement in the regeneration ability of older calli (16- to 20-week old) of all cultivars. Finally, exogenous application of 1 mM spermidine to 16-week old cultures, in combination with 16 h dehydration stress, improved plant regeneration by 10–65% in all nine cultivars.

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4 citations in Web of Science®

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ID Code: 10357
Item Type: Journal Article
Refereed: Yes
DOI: 10.1071/PP01115
ISSN: 1445-4408
Divisions: Past > QUT Faculties & Divisions > Faculty of Science and Technology
Copyright Owner: Copyright 2001 CSIRO Publishing
Deposited On: 23 Oct 2007 00:00
Last Modified: 15 Jan 2009 07:51

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