Vitamin B12 stalls the 80 S ribosomal complex on the hepatitis C internal ribosome entry site
Takyar, Seyedtaghi S., Gowans, Eric J., & Lott, William B. (2002) Vitamin B12 stalls the 80 S ribosomal complex on the hepatitis C internal ribosome entry site. Journal of Molecular Biology, 319(1), pp. 1-8.
The effect of cyanocobalamin (CNCbl, vitamin B12) on hepatitis C virus internal ribosome entry site (HCV IRES)-dependent initiation of translation was studied by ribosomal toeprinting and sucrose gradient centrifugation analysis. These results suggested that CNCbl did not inhibit HCV IRES-dependent translation by a competitive binding mechanism. CNCbl allowed 80 S elongation complex formation on the mRNA, but stalled the initiation at that point, effectively trapping the 80 S ribosomal complexes on the HCV IRES. CNCbl had no effect on cap-dependent mRNA, consistent with the known mRNA specificity of this translational inhibitor. To help elucidate the mechanism, comparative data were collected for the well-characterised translation inhibitors cycloheximide and 5′-guanylyl-imidophosphate. Although CNCbl stalled HCV IRES-dependent translation at approximately the same step in initiation as cycloheximide, the mechanisms of these two inhibitors are distinct.
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|Item Type:||Journal Article|
|Divisions:||Past > QUT Faculties & Divisions > Faculty of Science and Technology|
|Copyright Owner:||Copyright 2002 Academic Press|
|Deposited On:||26 Oct 2007 00:00|
|Last Modified:||15 Jan 2009 07:52|
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