Vitamin B12 stalls the 80 S ribosomal complex on the hepatitis C internal ribosome entry site

Takyar, Seyedtaghi S., Gowans, Eric J., & Lott, William B. (2002) Vitamin B12 stalls the 80 S ribosomal complex on the hepatitis C internal ribosome entry site. Journal of Molecular Biology, 319(1), pp. 1-8.

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Abstract

The effect of cyanocobalamin (CNCbl, vitamin B12) on hepatitis C virus internal ribosome entry site (HCV IRES)-dependent initiation of translation was studied by ribosomal toeprinting and sucrose gradient centrifugation analysis. These results suggested that CNCbl did not inhibit HCV IRES-dependent translation by a competitive binding mechanism. CNCbl allowed 80 S elongation complex formation on the mRNA, but stalled the initiation at that point, effectively trapping the 80 S ribosomal complexes on the HCV IRES. CNCbl had no effect on cap-dependent mRNA, consistent with the known mRNA specificity of this translational inhibitor. To help elucidate the mechanism, comparative data were collected for the well-characterised translation inhibitors cycloheximide and 5′-guanylyl-imidophosphate. Although CNCbl stalled HCV IRES-dependent translation at approximately the same step in initiation as cycloheximide, the mechanisms of these two inhibitors are distinct.

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ID Code: 10447
Item Type: Journal Article
Refereed: Yes
DOI: 10.1016/S0022-2836(02)00300-5
ISSN: 0022-2836
Divisions: Past > QUT Faculties & Divisions > Faculty of Science and Technology
Copyright Owner: Copyright 2002 Academic Press
Deposited On: 26 Oct 2007 00:00
Last Modified: 15 Jan 2009 07:52

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