Mapping the 5' Ends of Banana Bunchy Top Virus Gene Transcripts

Valencia, Virginia H., Dugdale, Benjamin, Harding, Robert M., & Dale, James L. (2006) Mapping the 5' Ends of Banana Bunchy Top Virus Gene Transcripts. Archives of Virology, 152(3), pp. 615-620.

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Banana bunchy top virus (BBTV), a multi-component circular ssDNA virus, replicates via a dsDNA intermediate that also serves as a template for virion sense transcription. Seven virus-derived transcripts have been previously identified and analysed in BBTV-infected bananas by northern analysis and 3′ rapid amplification of cDNA ends (3′ RACE). In this study, we have used RNA ligase-mediated rapid amplification of 5′ cDNA ends (RLM-RACE) to complete the mapping of the BBTV gene transcripts and have now fully mapped the transcribed regions of each BBTV component and effectively defined the upstream regulatory region.

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5 citations in Scopus
5 citations in Web of Science®
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ID Code: 10532
Item Type: Journal Article
Refereed: Yes
DOI: 10.1007/s00705-006-0889-6
ISSN: 0304-8608
Divisions: Past > QUT Faculties & Divisions > Faculty of Science and Technology
Copyright Owner: Copyright 2006 Springer-Verlag
Copyright Statement: The original publication is available at SpringerLink
Deposited On: 31 Oct 2007 00:00
Last Modified: 29 Feb 2012 13:26

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