Mapping the 5' Ends of Banana Bunchy Top Virus Gene Transcripts
Valencia, Virginia H., Dugdale, Benjamin, Harding, Robert M., & Dale, James L. (2006) Mapping the 5' Ends of Banana Bunchy Top Virus Gene Transcripts. Archives of Virology, 152(3), pp. 615-620.
DOI: 10.1007/s00705-006-0889-6
Abstract
Banana bunchy top virus (BBTV), a multi-component circular ssDNA virus, replicates via a dsDNA intermediate that also serves as a template for virion sense transcription. Seven virus-derived transcripts have been previously identified and analysed in BBTV-infected bananas by northern analysis and 3′ rapid amplification of cDNA ends (3′ RACE). In this study, we have used RNA ligase-mediated rapid amplification of 5′ cDNA ends (RLM-RACE) to complete the mapping of the BBTV gene transcripts and have now fully mapped the transcribed regions of each BBTV component and effectively defined the upstream regulatory region.
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| ID Code: | 10532 |
|---|---|
| Item Type: | Journal Article |
| ISSN: | 0304-8608 |
| Divisions: | Past > QUT Faculties & Divisions > Faculty of Science and Technology |
| Copyright Owner: | Copyright 2006 Springer-Verlag |
| Copyright Statement: | The original publication is available at SpringerLink http://www.springerlink.com |
| Deposited On: | 31 Oct 2007 |
| Last Modified: | 02 Feb 2012 20:14 |
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