Confocal microscopic observations of the human cornea following overnight contact lens wear

Efron, Nathan, Mutalib, Haliza A., Perez-Gomez, Inma, & Koh, Hui Hiang (2002) Confocal microscopic observations of the human cornea following overnight contact lens wear. Clinical and Experimental Optometry, 85(3), pp. 149-155.

Abstract

Background: Striae and folds are observed with a slitlamp biomicroscope in the cornea following overnight contact lens wear. These phenomena are poorly understood. The aim of this study is to employ confocal microscopy to observe and document these and other morphological changes in the human cornea following overnight contact lens wear. Methods: Slitlamp biomicroscopy, slit-scanning confocal microscopy and ultrasonic pachometry were performed on both eyes of 13 subjects (3M, 10F, age 24 ± 3 years) before and after eight hours overnight wear of a -3.00 D Bausch & Lomb one day disposable soft contact lens (Dk/t = 15.1 x 10-9 {cm/sec} x {ml O2/ml x mmHg}) in one eye; the other non-lens-wearing eye acted as a control. Results: Following sleep, both corneas were swollen (lens-wearing eye 11.8 ± 3.8 per cent; control eye 2.1 ± 1.9 per cent) and the stroma of both corneas displayed an apparent reduction in keratocyte density (lens-wearing eye 21 per cent; control eye 10 per cent). Folds were observed with the slitlamp biomicroscope and long, straight, dark, orthogonal lines were observed with the confocal microscope, in the posterior stroma of the oedematous lens-wearing eyes. Such features were not observed in the control eyes. The keratocytes appeared less distinct with greater levels of corneal oedema. Conclusion: The apparent loss of keratocytes following overnight lens wear is an optical artefact that can be explained in terms of corneal oedema causing volumetric tissue expansion and a loss of optical clarity, which hampers keratocyte detection. These findings place the onus on researchers postulating a loss of stromal keratocytes following clinical interventions, such as contact lens wear, to account for the effects of oedema.

Impact and interest:

47 citations in Scopus
Search Google Scholar™

Citation counts are sourced monthly from Scopus and Web of Science® citation databases.

These databases contain citations from different subsets of available publications and different time periods and thus the citation count from each is usually different. Some works are not in either database and no count is displayed. Scopus includes citations from articles published in 1996 onwards, and Web of Science® generally from 1980 onwards.

Citations counts from the Google Scholar™ indexing service can be viewed at the linked Google Scholar™ search.

Full-text downloads:

376 since deposited on 16 Nov 2007
24 in the past twelve months

Full-text downloads displays the total number of times this work’s files (e.g., a PDF) have been downloaded from QUT ePrints as well as the number of downloads in the previous 365 days. The count includes downloads for all files if a work has more than one.

ID Code: 10765
Item Type: Journal Article
Refereed: Yes
Additional Information: The contents of this journal from 1998 to 2005 can be freely accessed online via the journal’s web page (see link).
Additional URLs:
Keywords: closed eye, confocal microscopy, contact lens, cornea, keratocytes, oedema
ISSN: 0816-4622
Subjects: Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > OPTOMETRY AND OPHTHALMOLOGY (111300)
Divisions: Current > Research Centres > Centre for Health Research
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright 2002 Optometrists Association Australia
Deposited On: 16 Nov 2007 00:00
Last Modified: 10 Aug 2011 16:49

Export: EndNote | Dublin Core | BibTeX

Repository Staff Only: item control page