Confocal microscopic observations of stromal keratocytes during extended contact lens wear

Efron, Nathan, Perez-Gomez, Inma, & Morgan, Philip B. (2002) Confocal microscopic observations of stromal keratocytes during extended contact lens wear. Clinical and Experimental Optometry, 85(3), pp. 156-160.

PDF (52kB)


Background: The aim of this study was to monitor changes in keratocyte density during extended contact lens wear and to explore the possible role of hypoxia and oedema in any changes observed. Methods: Twenty-three neophyte myopic subjects wore a high Dk/t lens (PureVision) in one eye and a low Dk/t lens (Acuvue 2) in the other eye on an extended wear basis for six months. Slit-scanning confocal microscopy and ultrasonic pachometry were performed on both eyes at baseline (before lens wear), after three and six months of lens wear, and one week after cessation of lens wear (the 'postcessation' visit). Results: No differences were established between the two lenses or between the three study visits for anterior stromal keratocyte density (KD). Posterior stromal KD was similar for the two lenses throughout the study. However, there was an overall drop in posterior KD of 14 per cent in both eyes at the six-month visit, compared to the initial visit. Posterior KD at the six-month visit was no different from that at the post-cessation visit. Corneal thickness was similar for the two lenses at the initial and post-cessation visits, but was three per cent greater for the eye wearing the Acuvue 2 lens at the six-month visit. Conclusion: Extended contact lens wear causes a loss of keratocytes; this loss cannot be attributed to contact lens-induced hypoxia and/or oedema, or to an artifact of confocal microscopy relating to presence of residual oedema. It is postulated that lens-induced keratocyte loss may be related to the physical presence of contact lenses creating some mechanical effect.

Impact and interest:

41 citations in Scopus
Search Google Scholar™

Citation counts are sourced monthly from Scopus and Web of Science® citation databases.

These databases contain citations from different subsets of available publications and different time periods and thus the citation count from each is usually different. Some works are not in either database and no count is displayed. Scopus includes citations from articles published in 1996 onwards, and Web of Science® generally from 1980 onwards.

Citations counts from the Google Scholar™ indexing service can be viewed at the linked Google Scholar™ search.

Full-text downloads:

289 since deposited on 16 Nov 2007
28 in the past twelve months

Full-text downloads displays the total number of times this work’s files (e.g., a PDF) have been downloaded from QUT ePrints as well as the number of downloads in the previous 365 days. The count includes downloads for all files if a work has more than one.

ID Code: 10766
Item Type: Journal Article
Refereed: Yes
Additional Information: The contents of this journal from 1998 to 2005 can be freely accessed online via the journal’s web page (see link).
Additional URLs:
Keywords: confocal microscopy, contact lens, cornea, extended wear, keratocytes, oedema
ISSN: 0816-4622
Subjects: Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > OPTOMETRY AND OPHTHALMOLOGY (111300)
Divisions: Current > Research Centres > Centre for Health Research
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright 2002 Optometrists Association Australia
Deposited On: 16 Nov 2007 00:00
Last Modified: 10 Aug 2011 16:49

Export: EndNote | Dublin Core | BibTeX

Repository Staff Only: item control page