A population study of the normal cornea using an in vivo, slit-scanning confocal microscope

Hollingsworth, Joanna G., Perez-Gomez, Inma, Mutalib, Haliza A., & Efron, Nathan (2001) A population study of the normal cornea using an in vivo, slit-scanning confocal microscope. Optometry And Vision Science, 78(10), pp. 706-711.


PURPOSE: To document qualitative and quantitative changes in the normal, healthy human cornea with age using the confocal microscope. METHODS: The central corneas of 120 subjects (mean age, 41 years; range, 11 to 80 years) were examined using an in vivo slit-scanning real-time confocal microscope. Images of the corneal stroma and endothelium from both eyes of each subject were semiautomatically analyzed in an observer-masked, randomized manner. RESULTS: Anterior keratocyte density, posterior keratocyte density, and endothelial cell density were shown to be unaffected by the sex of the subject with p values of 0.46, 0.55, 0.50, respectively (multivariate analysis of variance). No statistically significant difference was detected between right and left eyes for all corneal layers examined. The anterior keratocyte density, posterior keratocyte density, and endothelial cell density decreased at a rate of 0.48, 0.22, and 0.33% per year, respectively. A positive correlation was found between the coefficient of cell variation and age. CONCLUSIONS: This data constitutes essential normative data that can be used as a control in further research into abnormal corneal conditions.

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ID Code: 11009
Item Type: Journal Article
Refereed: Yes
Additional Information: Self-archiving of the author-version is not yet supported by this publisher. For more information, please refer to the journal’s website or contact the author. NOTE: The contents of this journal can be freely accessed online via the journal's web page (see hypertext link) 12 months after publication.
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Keywords: confocal microscopy, normal cornea, keratocyte density, endothelial cell density
ISSN: 1040-5488
Subjects: Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > OPTOMETRY AND OPHTHALMOLOGY (111300)
Divisions: Current > Research Centres > Centre for Health Research
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright 2001 Lippincott Williams & Wilkins
Deposited On: 28 Nov 2007 00:00
Last Modified: 10 Aug 2011 16:49

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