23Na NMR micro-imaging – a tool for non-invasive monitoring of sodium distribution in living plants

Rokitta, Markus, Medek, Danielle, Pope, James M., & Critchley, Christa (2004) 23Na NMR micro-imaging – a tool for non-invasive monitoring of sodium distribution in living plants. Functional Plant Biology, 31(9), pp. 879-887.

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Detailed knowledge of the sodium distribution within the tissues of Australian native species that are highly salt tolerant could help in understanding the physiological adaptation to salt tolerance or sensitivity. 23Na NMR microimaging is presented as a tool to achieve this goal. Maps of the sodium distribution in stem tissue were obtained with an in-plane resolution of approximately 125 µm and a slice thickness of 4 mm. Simultaneously recorded high resolution 1H NMR images, showing water distribution in the same slice with 31 µm in-plane resolution and 1 mm slice thickness, were used as an anatomical reference together with optical micrographs which were taken immediately after the NMR experiments were completed. To quantify the sodium concentration reference capillaries with known NaCl concentration were located in the NMR probe together with the plant sample. Average concentration values calculated from signal intensities in the tissue and the capillaries were compared with concentration values obtained from atomic emission photometry, performed on digested stem sections harvested immediately after NMR experiments and with optical microscopy. This shows that 23Na NMR microimaging has great potential for physiological studies of salt stress at the macroscopic level and may become a unique tool for diagnosing salinity tolerance and sensitivity

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ID Code: 1105
Item Type: Journal Article
Refereed: Yes
Additional URLs:
Keywords: Environmental stress, NMR micro, imaging, Plant water relations, Salinity, 23Na MRI
ISSN: 1445-4408
Subjects: Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > PLANT BIOLOGY (060700) > Plant Physiology (060705)
Australian and New Zealand Standard Research Classification > ENVIRONMENTAL SCIENCES (050000) > SOIL SCIENCES (050300) > Land Capability and Soil Degradation (050302)
Australian and New Zealand Standard Research Classification > PHYSICAL SCIENCES (020000) > OTHER PHYSICAL SCIENCES (029900) > Biological Physics (029901)
Divisions: Past > QUT Faculties & Divisions > Faculty of Science and Technology
Copyright Owner: Copyright 2004 Csiro Publishing
Copyright Statement: Reproduced in accordance with the copyright policy of the publisher.
Deposited On: 05 Jul 2005 00:00
Last Modified: 29 Feb 2012 13:08

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