Performance evaluation of the UVAPS: influence of physiological age of airborne bacteria and bacterial stress
Agranovski, Victoria, Ristovski, Zoran, Hargreaves, Megan, Blackall, Patrick J. , & Morawska, Lidia (2003) Performance evaluation of the UVAPS: influence of physiological age of airborne bacteria and bacterial stress. Journal of Aerosol Science, 34(12), pp. 1711-1727.
This study evaluated the effect of bacterial physiology, such as physiological age and stress, on the performance of the Ultravioloet Aerodynamic Particle Sizer (UV-APS, model 3312, TSI Inc., St. Paul, MN). Intensity of the fluorescent signals was measured for three bacteria having various sensitivities to environmental stresses, Bacillus subtilus (spores and vegetative cells), Pseudomonas fluorescens, and Micrococcus luteus. The performance of the UVAPS was found to depend on the type of airborne bacteria. In addition, the fluorescence signals for stationary-phase bacteria were generally stronger than for their log-phase counterparts. These results indicated that bacterial injury due to environmental stresses has a strong influence on the measured fluorescence signals. This hypothesis was confirmed by obtaining a linear relationship between the percentage of fluorescent particles and the proportion of injured bacteria in the total population of cultivable bacteria in samples simultaneously collected with the AGI-30 impingers. This indicates that the amount of fluorophors (specifically NADH) within injured bacteria is below the UVAPS sensitivity level. The practical implications of these findings are discussed in the paper. The reported results contribute to broadening our understanding of the method and may assist in developing sampling strategies for the application of the UVAPS to various bioaerosol studies.
Impact and interest:
Citation countsare sourced monthly fromand citation databases.
These databases contain citations from different subsets of available publications and different time periods and thus the citation count from each is usually different. Some works are not in either database and no count is displayed. Scopus includes citations from articles published in 1996 onwards, and Web of Science® generally from 1980 onwards.
Citations counts from theindexing service can be viewed at the linked Google Scholar™ search.
Full-text downloadsdisplays the total number of times this work’s files (e.g., a PDF) have been downloaded from QUT ePrints as well as the number of downloads in the previous 365 days. The count includes downloads for all files if a work has more than one.
|Item Type:||Journal Article|
|Keywords:||Bacterial aerosols, Real, time monitoring, Intrinsic fluorescence, Performance evaluation|
|Subjects:||Australian and New Zealand Standard Research Classification > EARTH SCIENCES (040000) > ATMOSPHERIC SCIENCES (040100)|
Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > MICROBIOLOGY (060500)
|Divisions:||Past > QUT Faculties & Divisions > Faculty of Science and Technology|
|Copyright Owner:||Copyright 2003 Elsevier|
|Copyright Statement:||Reproduced in accordance with the copyright policy of the publisher.|
|Deposited On:||04 May 2005|
|Last Modified:||29 Feb 2012 22:58|
Repository Staff Only: item control page