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Hyperbaric oxygen stimulates epidermal reconstruction in human skin equivalents

Kairuz, Evette, Upton, Zee, Dawson, Rebecca A., & Malda, Jos (2007) Hyperbaric oxygen stimulates epidermal reconstruction in human skin equivalents. Wound Repair and Regeneration, 15(2), pp. 266-274.

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Abstract

The crucial role of oxygen during the complex process of wound healing has been extensively described. In chronic or nonhealing wounds, much evidence has been reported indicating that a lack of oxygen is a major contributing factor. Although still controversial, the therapeutic application of hyperbaric oxygen (HBO) therapy can aid the healing of chronic wounds. However, how HBO affects reepithelization, involving processes such as keratinocyte proliferation and differentiation, remains unclear. We therefore used a three-dimensional human skin-equivalent (HSE) model to investigate the effects of daily 90-minute HBO treatments on the reconstruction of an epidermis. Epidermal markers of proliferation, differentiation, and basement membrane components associated with a developing epidermis, including p63, collagen type IV, and cytokeratins 6, 10, and 14, were evaluated. Morphometric analysis of hematoxylin and eosin-stained cross sections revealed that HBO treatments significantly accelerated cornification of the stratum corneum compared with controls. Protein expression as determined by immunohistochemical analysis confirmed the accelerated epidermal maturation. In addition, early keratinocyte migration was enhanced by HBO. Thus, HBO treatments stimulate epidermal reconstruction in an HSE. These results further support the importance of oxygen during the process of wound healing and the potential role of HBO therapy in cutaneous wound healing.

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17 citations in Scopus
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13 citations in Web of Science®

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ID Code: 12322
Item Type: Journal Article
Keywords: Cell Differentiation, Cell Proliferation, Collagen Type IV, Epidermis, Hyperbaric 0xygenation, Immunohistochemistry, Keratin, 14, Keratinocytes, Keratins, metabolism, Time Factors, Wound Healing
DOI: 10.1111/j.1524-475X.2007.00215.x
ISSN: 1067-1927
Subjects: Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > CLINICAL SCIENCES (110300) > Dermatology (110304)
Divisions: Past > QUT Faculties & Divisions > Faculty of Science and Technology
Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright 2007 Blackwell Publishing
Copyright Statement: The definitive version is available at www.blackwell-synergy.com
Deposited On: 05 Feb 2008
Last Modified: 29 Feb 2012 23:39

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