Implications of the Variability in Time to Isotopic Equilibrium in the Deuterium Dilution Technique

Colley, Rachel C., Byrne, Nuala M., & Hills, Andrew P. (2007) Implications of the Variability in Time to Isotopic Equilibrium in the Deuterium Dilution Technique. European Journal of Clinical Nutrition, 61(11), p. 1250.

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OBJECTIVE: To investigate the variability in isotopic equilibrium time under field conditions, and the impact of this variability on estimates of total body water (TBW) and body composition. DESIGN AND SETTING: Following collection of a fasting baseline urine sample, 10 women and 10 men were dosed with deuterium oxide (0.05 g/kg body weight). Urine samples were collected every hour for 8 h. The samples were analysed using isotope ratio mass spectrometry. Time to equilibration was determined using three commonly employed data analysis approaches. RESULTS: Isotopic equilibrium was reached by 50, 80 and 100% of participants at 4, 6 and 8 h, respectively. The mean group equilibration determined using the three different plateau determination methods were 4.8+/-1.5, 3.8+/-0.8 and 4.9+/-1.4 h. Isotopic enrichment, TBW, and percent body fat estimates differed between early (3-5 h), but not later sampling times (5-8 h). CONCLUSION: Although the three different plateau determination approaches resulted in differences in equilibration time, all suggest that sampling at 6 h or later will decrease the likelihood of error in body composition estimates resultant from incomplete isotopic equilibration in a small proportion of individuals.

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13 citations in Web of Science®
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ID Code: 12723
Item Type: Journal Article
Refereed: Yes
Additional Information: For more information, please refer to the journal's website (see hypertext link) or contact the author.
Keywords: total body water, body composition, urine, sampling time
DOI: 10.1038/sj.ejcn.1602653
ISSN: 0954-3007
Subjects: Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > HUMAN MOVEMENT AND SPORTS SCIENCE (110600)
Divisions: Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright 2007 Nature Publishing Group
Deposited On: 02 Sep 2008 00:00
Last Modified: 09 Apr 2014 12:21

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