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Biomolecular hydrogels formed and degraded via site-specific enzymatic reactions

Ehrbar, Martin, Rizzi, Simone C., Schoenmakers, Ronald G., San Miguel, Blanca, Hubbell, Jeffrey A., Weber, Franz E., & Lutolf, Matthias P. (2007) Biomolecular hydrogels formed and degraded via site-specific enzymatic reactions. Biomacromolecules, 8(10), pp. 3000-3007.

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Abstract

We present polymeric hydrogel biomaterials that are biomimetic both in their synthesis and degradation. The design of oligopeptide building blocks with dual enzymatic responsiveness allows us to create polymer networks that are formed and functionalized via enzymatic reactions and are degradable via other enzymatic reactions, both occurring under physiological conditions. The activated transglutaminase enzyme factor XIIIa was utilized for site-specific coupling of prototypical cell adhesion ligands and for simultaneous cross-linking of hydrogel networks from factor XIIIa substrate-modified multiarm poly(ethylene glycol) macromers. Ligand incorporation is nearly quantitative and thus controllable, and does not alter the network's macroscopic properties over a concentration range that elicits specific cell adhesion. Living mammalian cells can be encapsulated in the gels without any noticeable decrease in viability. The degradation of gels can be engineered to occur, for example, via cell-secreted matrix metalloproteinases, thus rendering these gels interesting for biomedical applications such as drug delivery systems or smart implants for in situ tissue engineering.

Impact and interest:

93 citations in Scopus
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82 citations in Web of Science®

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ID Code: 12895
Item Type: Journal Article
Additional Information: This article is freely available from the American Chemical Society website 12 months after the publication date. See links to publisher website in this record.
Keywords: matrix metalloproteinases, tissue repair and regeneration, ihbi, cells and tissue, trr
DOI: 10.1021/bm070228f
ISSN: 1525-7797
Subjects: Australian and New Zealand Standard Research Classification > CHEMICAL SCIENCE (030000) > MACROMOLECULAR AND MATERIALS CHEMISTRY (030300) > Chemical Characterisation of Materials (030301)
Australian and New Zealand Standard Research Classification > CHEMICAL SCIENCE (030000) > MACROMOLECULAR AND MATERIALS CHEMISTRY (030300)
Divisions: Past > QUT Faculties & Divisions > Faculty of Science and Technology
Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright 2007 American Chemical Society
Deposited On: 06 Mar 2008
Last Modified: 29 Feb 2012 23:39

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