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The preclinical testing of a formaldehyde inactivated Ross River virus vaccine designed for use in humans

Kistnera, Otfried, Barretta, Noel, Bruhmanna, Axel, Reitera, Manfred, Mundta, Wolfgang, Savidis-Dachoa, Helga, Schober-Bendixen, Susanne, Dorner, Friedrich, & Aaskov, John G. (2007) The preclinical testing of a formaldehyde inactivated Ross River virus vaccine designed for use in humans. Vaccine, 25(25), pp. 4845-4852.

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Abstract

Ross River virus was grown in industrial facilities in vaccine-certified Vero cells in the absence of serum, inactivated using standard formalin-inactivation protocols, treated with Benzonase to digest host cell DNA and purified on a sucrose gradient. Mice given two subcutaneous injections of 0.625 microg of this vaccine or two doses of 0.156 microg vaccine with aluminium hydroxide adjuvant failed to develop a detectable viraemia after intravenous challenge with 10(6)TCID50 of the prototype strain of Ross River virus (T48). Guinea pigs immunised with one or two10 microg doses of vaccine with adjuvant also failed to develop a detectable viraemia following a similar challenge. The levels of neutralising antibody (neutralisation index 1.9-3.1) in the mice protected against challenge with 10(6)TCID50 Ross River virus were similar to those in 16 former epidemic polyarthritis patients (1.1-3.5) who had not experienced a second clinical infection with Ross River virus in the 20 years following their initial infection.

Impact and interest:

21 citations in Scopus
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13 citations in Web of Science®

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ID Code: 13701
Item Type: Journal Article
Additional Information: For more information, please refer to the journal's website (see hypertext link) or contact the author.
Keywords: infectious diseases, cells and tissue, ihb, Ross River virus, Vaccine, Epidemic polyarthritis
DOI: 10.1016/j.vaccine.2007.01.103
ISSN: 0264-410X
Subjects: Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > MICROBIOLOGY (060500) > Microbiology not elsewhere classified (060599)
Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > MICROBIOLOGY (060500) > Infectious Agents (060502)
Divisions: Past > QUT Faculties & Divisions > Faculty of Science and Technology
Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright 2007 Elsevier
Deposited On: 10 Jun 2008
Last Modified: 05 Feb 2013 12:30

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