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Functional analysis of the multi-copper oxidase from Legionella pneumophila

Huston, Wilhelmina M., Naylor, Jennifer, Cianciotto, Nicholas P., Jennings, Michael P., & McEwan, Alastair G. (2008) Functional analysis of the multi-copper oxidase from Legionella pneumophila. Microbes and Infection, 10(5), pp. 497-503.

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Abstract

Multicopper oxidases have been described to have functions in copper tolerance, manganese oxidation, and iron oxidation in a range of bacteria. The putative cytoplasmic membrane multicopper oxidase from Legionella pneumophila was investigated. The mcoL gene was found to be critical for aerobic extracellular growth under either iron-limiting conditions or in the presence of ferrous Fe(II) iron, as a sole source of this essential metal. The mcoL mutants showed minor growth defects when grown in the presence of Fe(III) as the iron source. In contrast, intracellular growth and survival was not affected by the absence of the mcoL gene regardless of available iron concentration. The evidence presented here could indicate a possible role for mcoL in prevention of the toxic effects of ferrous iron during aerobic conditions. However, a function in high-affinity acquisition of iron could also be possible given the inability of the McoL mutants to grow aerobically under iron-limiting conditions.

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7 citations in Scopus
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6 citations in Web of Science®

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ID Code: 13702
Item Type: Journal Article
Additional Information: For more information, please refer to the journal's website (see hypertext link) or contact the author.
Keywords: Legionella, Multicopper oxidase, Iron, infectious diseases, cells and tissue, ihbi
DOI: 10.1016/j.micinf.2008.01.011
ISSN: 0022-1317
Subjects: Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > MICROBIOLOGY (060500) > Microbiology not elsewhere classified (060599)
Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > MICROBIOLOGY (060500) > Infectious Agents (060502)
Divisions: Past > QUT Faculties & Divisions > Faculty of Science and Technology
Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright 2008 Elsevier
Deposited On: 10 Jun 2008
Last Modified: 01 Mar 2012 00:25

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