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Structural changes in the cells of some bacteria during population growth: An FT-IR/ATR study

Ede, Sarah M., Hafner, Louise M., & Fredericks, Peter M. (2004) Structural changes in the cells of some bacteria during population growth: An FT-IR/ATR study. Applied Spectroscopy, 53(3), pp. 317-322.

Abstract

Structural changes occurring in the cells of several bacteria during their growth curves have been investigated by Fourier transform infrared (FT-IR) spectroscopy using the sampling technique of attenuated total reflectance (ATR). Spectra reflect all of the components of the cells including the cell walls, cell membranes, internal structures and the cytoplasm. The bacteria studied were Bacillus stearothermophilus, Halobacterium salinarium, Halococcus morrhuae and Acetobacter aceti. All species showed significant spectral changes during their growth curves, indicating structural changes in the cells during increases in cell numbers. The major change for B. stearothermophilus was in the lipid content which was at a maximum during the exponential phase of the growth curve. For the halophiles H. salinarium and H. morrhuae the major change was that the concentration of sulfate ion in the cells varied during the growth curve and was at a maximum during the mid-part of the exponential phase of the growth curve. A. aceti cells showed increasing polysaccharide content during the growth curve as well as maximum lipid content during the exponential phase of growth.

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ID Code: 1396
Item Type: Journal Article
Additional URLs:
Keywords: Infrared spectroscopy, FTIR, ATR, bacteria, growth curve
ISSN: 0003-7028
Subjects: Australian and New Zealand Standard Research Classification > CHEMICAL SCIENCE (030000) > PHYSICAL CHEMISTRY (INCL. STRUCTURAL) (030600) > Structural Chemistry and Spectroscopy (030606)
Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > MICROBIOLOGY (060500) > Bacteriology (060501)
Australian and New Zealand Standard Research Classification > CHEMICAL SCIENCE (030000) > ANALYTICAL CHEMISTRY (030100) > Instrumental Methods (excl. Immunological and Bioassay Methods) (030105)
Australian and New Zealand Standard Research Classification > CHEMICAL SCIENCE (030000) > ANALYTICAL CHEMISTRY (030100) > Analytical Spectrometry (030101)
Divisions: Past > QUT Faculties & Divisions > Faculty of Science and Technology
Copyright Owner: Copyright 2004 Society for Applied Spectroscopy
Copyright Statement: Reproduced in accordance with the copyright policy of the publisher.
Deposited On: 13 Oct 2005
Last Modified: 29 Feb 2012 23:52

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