Isolated HIV-1 core is active for reverse transcription
Warrilow, David, Stenzel, Deborah J., & Harrich, David (2007) Isolated HIV-1 core is active for reverse transcription. Retrovirology, 4(77).
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Abstract
Whether purified HIV-1 virion cores are capable of reverse transcription or require uncoating to be activated is currently controversial. To address this question we purified cores from a virus culture and tested for the ability to generate authentic reverse transcription products. A dense fraction (approximately 1.28 g/ml) prepared without detergent, possibly derived from disrupted virions, was found to naturally occur as a minor sub-fraction in our preparations. Core-like particles were identified in this active fraction by electron microscopy. We are the first to report the detection of authentic strong-stop, first-strand transfer and full-length minus strand products in this core fraction without requirement for an uncoating activity.
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| ID Code: | 14180 |
|---|---|
| Item Type: | Journal Article |
| DOI: | 10.1186/1742-4690-4-77 |
| ISSN: | 1742-4690 |
| Subjects: | Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > MICROBIOLOGY (060500) > Virology (060506) |
| Divisions: | Current > QUT Faculties and Divisions > Faculty of Health Current > Institutes > Institute of Health and Biomedical Innovation |
| Copyright Owner: | Copyright 2007 (The authors); licensee BioMed Central Ltd. |
| Copyright Statement: | © 2007 Warrilow et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| Deposited On: | 28 Jul 2008 |
| Last Modified: | 29 Feb 2012 23:38 |
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