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Anisotropy of collagen fibre alignment in bovine cartilage: Comparison of polarised light microscopy and spatially-resolved diffusion-tensor measurements

de Visser, Sally K., Bowden, Joshua C., Wentrup-Byrne, Edeline, Rintoul, Llew, Bostrom, Thor E., Pope, James M., & Momot, Konstantin I. (2008) Anisotropy of collagen fibre alignment in bovine cartilage: Comparison of polarised light microscopy and spatially-resolved diffusion-tensor measurements. Osteoarthritis and Cartilage, 16(6), pp. 689-697.

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Abstract

Objective: To compare collagen fibre alignment angles obtained from polarised light microscopy and diffusion tensor imaging in bovine articular cartilage.

Methods: Five samples of bovine articular cartilage from five different animals were studied using MRI and PLM techniques. T2-weighted, diffusion-tensor, and PLM images were acquired for each sample and average depth profiles of the PLM and DTI angles, as well as the banding patterns observed in T2-weighted MR images, were compared. Statistical properties of the distributions of the DTI and PLM angles were examined.

Results: The samples exhibited a range of alignment morphologies. In the samples with the "conventional" three-zone alignment pattern, a correlation between the PLM and DTI alignment zones and the banding in T2-weighted MR images was observed. The shapes of the depth profiles of the PLM and DTI alignment angles were qualitatively similar for each sample. Three samples showed good quantitative correlation between the diffusion-tensor and PLM alignment angles. The correlation between the diffusion and PLM alignment angles was best in the regions of low degree of disorder of fibre alignment.

Conclusions: This study provides the first quantitative comparison of DTI of cartilage with the more established PLM techniques. The correlation between alignment angles derived from PLM and DTI data was evident across a wide range of alignment morphologies. The results support the use of DTI for the quantitative measurement of collagen fibre alignment. The microscopic-scale (~10 um) dispersion of fibre alignment angles appears to be an important factor for understanding the extent of quantitative correlation between PLM and DTI results.

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27 citations in Web of Science®

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ID Code: 14381
Item Type: Journal Article
Keywords: Collagen fibre orientation, Diffusion, tensor imaging, Polarised light microscopy, Anisotropy, Birefringence, Fibre alignment order
DOI: 10.1016/j.joca.2007.09.015
ISSN: 1063-4584
Subjects: Australian and New Zealand Standard Research Classification > PHYSICAL SCIENCES (020000) > OTHER PHYSICAL SCIENCES (029900) > Biological Physics (029901)
Divisions: Past > QUT Faculties & Divisions > Faculty of Science and Technology
Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright 2008 Elsevier
Copyright Statement: Reproduced in accordance with the copyright policy of the publisher.
Deposited On: 14 Aug 2008
Last Modified: 25 Feb 2013 13:49

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