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Sequencing Aided by Mutagenesis (SAM) Facilitates the De Novo Sequencing of Megabase DNA Fragments by Short Read Lengths

Keith, Macgregor, Hawkes, David B., Carter, Jacinta C., Cochran, Duncan A.E., Adams, Peter , Bryant, Darryn E., & Mitchelson, Keith R. (2007) Sequencing Aided by Mutagenesis (SAM) Facilitates the De Novo Sequencing of Megabase DNA Fragments by Short Read Lengths. In Mitchelson, Keith (Ed.) New High Throughput Technologies for DNA Sequencing and Genomics, Volume 2. Elsevier, Burlington, pp. 303-326.

Abstract

Since the independent invention of DNA sequencing by Sanger and by Gilbert 30 years ago, it has grown from a small scale technique capable of reading several kilobase-pair of sequence per day into today's multibillion dollar industry. This growth has spurred the development of new sequencing technologies that do not involve either electrophoresis or Sanger sequencing chemistries. Sequencing by Synthesis (SBS) involves multiple parallel micro-sequencing addition events occurring on a surface, where data from each round is detected by imaging. New High Throughput Technologies for DNA Sequencing and Genomics is the second volume in the Perspectives in Bioanalysis series, which looks at the electroanalytical chemistry of nucleic acids and proteins, development of electrochemical sensors and their application in biomedicine and in the new fields of genomics and proteomics. The authors have expertly formatted the information for a wide variety of readers, including new developments that will inspire students and young scientists to create new tools for science and medicine in the 21st century. Reviews of complementary developments in Sanger and SBS sequencing chemistries, capillary electrophoresis and microdevice integration, MS sequencing and applications set the framework for the book.

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ID Code: 15244
Item Type: Book Chapter
Additional Information: For more information about this book please refer to the publisher's website (see link) or contact the author.
Additional URLs:
ISBN: 9780444522238
Subjects: Australian and New Zealand Standard Research Classification > TECHNOLOGY (100000) > MEDICAL BIOTECHNOLOGY (100400) > Medical Molecular Engineering of Nucleic Acids and Proteins (100403)
Divisions: Past > QUT Faculties & Divisions > Faculty of Science and Technology
Copyright Owner: Copyright 2007 Elsevier
Deposited On: 20 Oct 2008
Last Modified: 29 Feb 2012 23:29

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