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Osteogenic and adipogenic induction potential of human periodontal cells

Zhou, Yefang, Hutmacher, Dietmar W., Sae-Lim, Varawan, Zhou, Zhihong, Woodruff, Maria A., & Lim, Tit Meng (2008) Osteogenic and adipogenic induction potential of human periodontal cells. Journal Of Periodontology, 79(3), pp. 525-534.

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Abstract

BACKGROUND: Human periodontium contains different cell types that have various potential roles in hard and soft tissue regeneration. However, there is limited knowledge about how these diverse cell populations contribute to the regenerative process. In this study, we investigated the surface marker difference between different periodontal cells (alveolar osteoblasts [AOs], periodontal ligament fibroblasts [PDLFs], and gingival fibroblasts [GFs]) and their differentiation potential toward osteogenic and adipogenic phenotypes. METHODS: Periodontal cells (AOs, PDLFs, and GFs) from 14 subjects were isolated. The surface antigen expression pattern of cells was analyzed by cell flow cytometry, and the molecular and histologic characterizations under osteogenic and adipogenic inductions were monitored by reverse transcription-polymerase chain reaction, Western blot, and immunocytohistology. RESULTS: The cell phenotypes of AOs were verified by the high expressions of CD29 and CD49a, whereas PDLFs showed distinctively low levels of CD63 and CD73. Under adipogenic induction, limited AOs formed cube-shaped adipose-like cells, whereas PDLFs formed spindle-shaped adipose-like cells. All three cell types expressed baseline osteo-related genes. AOs demonstrated the highest osteogenic ability followed by PDLFs and GFs. CONCLUSIONS: Cells in alveolar bone and periodontal ligament contain osteogenic and adipogenic progenitors. These observations indicate a possible application for periodontium cells in hard or soft tissue regeneration.

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19 citations in Scopus
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18 citations in Web of Science®

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ID Code: 15411
Item Type: Journal Article
Additional Information: For more information, please refer to the journal's website (see hypertext link) or contact the author.
Keywords: Adipogenesis, genetics, Osteogenesis, Adult Stem Cells, Alveolar Process, Periodontal Ligament, Adipocytes, Alveolar Process, Antigens, Calcification, Physiologic, Cell Differentiation, Fibroblasts, Gene Expression
DOI: 10.1902/jop.2008.070373
ISSN: 0022-3492
Subjects: Australian and New Zealand Standard Research Classification > ENGINEERING (090000) > BIOMEDICAL ENGINEERING (090300) > Rehabilitation Engineering (090305)
Divisions: Past > QUT Faculties & Divisions > Faculty of Built Environment and Engineering
Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright 2008 American Academy of Periodontology
Deposited On: 31 Oct 2008
Last Modified: 29 Feb 2012 23:50

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