The role of urothelium in induced ossification in skeletal muscle
Podagiel, Christopher (2006) The role of urothelium in induced ossification in skeletal muscle. Masters by Research thesis, Queensland University of Technology.
It is a well established phenomenon that the epithelial lining of the urinary bladder
(urothelium) when implanted into skeletal muscle induces ectopic ossification. However,
despite numerous observations, this reaction is poorly understood. This research further
studied this reaction by - (a) demonstrating the reaction in a suitable small animal model;
(b) attempting to induce the reaction by implanting urothelial cells purified by cell
culture techniques; and (c) comparing the bone forming reaction induced by implanted urothelium to the reaction induced by implanting Bone Marrow Stem Cells (BMSC's) and Osteophyte Stem Cells (OSC's). By demonstrating newly formed bone after the implantation of guinea pig urothelium into the skeletal muscle of a Severe Combined Immuno-Deficient Mouse (SCID-Mouse) this research demonstrated that a suitable small animal model had been established. This is despite inherent difficulties (particularly bacterial contamination) associated with establishing a primary cell culture of guinea pig urothelial cells. Additionally, the intramuscular ectopic osteoinductive potential of human BMSC's (hBMSC's) in the SCID-mouse has also been demonstrated. Confirming that the injection of cultured cells in suspension is an adequate intramuscular delivery technique, this research demonstrates that hBMSC's induce ectopic ossification by non-immunological means. This research has demonstrated a number of differences between urothelium induced ectopic ossification and ectopic ossification induced by BMSC's, suggesting they are two separate processes. This is important because the chemotaxis and subsequent osteogenic differentiation of BMSC's has previously been one of the more popular postulated mechanisms of urothelium induced ectopic ossification. Finally, this research has demonstrated the ectopic osteoinductive potential of stem cells isolated from the marrow of human osteophytes (human Osteophyte Stem Cells, hOSC's). This observation has not been previously reported, and will hopefully provide a valuable contribution to a body of knowledge that has important ramifications in both the treatment of osteoarthritis, and the use of BMSC's in tissue engineering.
Citation countsare sourced monthly fromand citation databases.
These databases contain citations from different subsets of available publications and different time periods and thus the citation count from each is usually different. Some works are not in either database and no count is displayed. Scopus includes citations from articles published in 1996 onwards, and Web of Science generally from 1980 onwards.
Citations counts from theindexing service can be viewed at the linked Google Scholar™ search.
Full-text downloadsdisplays the total number of times this work’s files (e.g., a PDF) have been downloaded from QUT ePrints as well as the number of downloads in the previous 365 days. The count includes downloads for all files if a work has more than one.
|Item Type:||QUT Thesis (Masters by Research)|
|Supervisor:||Goss, Benjamin, Crawford, Ross, Williams, Richard, & Xiao, Yin|
|Keywords:||urothelium, ectopic ossification, skeletal muscle, epithelial mesenchymal transition, bone marrow stem cell|
|Divisions:||Past > QUT Faculties & Divisions > Faculty of Built Environment and Engineering|
Past > Schools > School of Engineering Systems
|Department:||Faculty of Built Environment and Engineering|
|Institution:||Queensland University of Technology|
|Copyright Owner:||Copyright Christopher Podagiel|
|Deposited On:||03 Dec 2008 14:02|
|Last Modified:||29 Oct 2011 05:46|
Repository Staff Only: item control page