Bovine serum albumin adhesion force measurements using an atomic force microscopy

Lai, Chun-Chih (2006) Bovine serum albumin adhesion force measurements using an atomic force microscopy. Masters by Research thesis, Queensland University of Technology.

Abstract

In this thesis, a direct method of Atomic Force Microscopy (AFM) technique has been developed to measure the adhesion forces between BSA and two different surfaces: mica (a hydrophilic surface); and polystyrene (a hydrophobic surface); in PBS solution. We have shown possible to measure interactions between proteins and substrate surface directly without any modification to the substrate and the AFM tip; this means protein molecules can keep the natural elastic property within the force measurements. The average measured value of adhesion forces between BSA and mica is 0.036 ± 0.002 nN, and between BSA and polystyrene is 0.066 ± 0.003 nN. The polystyrene surface is more adhesive to BSA than the mica surface. This is consistent with previous research, which assessed that hydrophobic surfaces enhance protein adhesion but hydrophilic surfaces do not.

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ID Code: 16418
Item Type: QUT Thesis (Masters by Research)
Supervisor: Bell, John & Wentrup-Byrne, Edeline
Keywords: Atomic Force Microscopy (AFM) technique, hydrophobic surfaces, protein adhesion, hydrophilic surfaces, Bovine Serum Albumin adhesion, measuring adhesion forces
Divisions: Past > QUT Faculties & Divisions > Faculty of Built Environment and Engineering
Past > Schools > School of Engineering Systems
Department: Faculty of Built Environment and Engineering
Institution: Queensland University of Technology
Copyright Owner: Copyright Chun-Chih Lai
Deposited On: 03 Dec 2008 04:03
Last Modified: 02 Feb 2012 09:34

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