Segmentation of bone marrow stromal cells in phase contrast microscopy images
Bradhurst, Christopher J., Boles, Wageeh W., & Xiao, Yin (2008) Segmentation of bone marrow stromal cells in phase contrast microscopy images. In International Conference Image and Vision Computing 2008, 25-28 November 2008, Christchurch, New Zealand.
The morphology of bone marrow stromal cells (BMSCs) and the nature of phase contrast images make segmentation of such images challenging as many standard segmentation approaches do not work. This presents an obstacle to the development of systems that could use pattern recognition (PR) techniques to assess culture quality, since successful segmentation is an important precursor to successful pattern recognition. A method is presented for image normalisation and segmentation of cell regions within sub-confluent cell cultures of human bone marrow stromal cells, including a novel method of dealing with the halo associated with phase contrast images. The proposed method was evaluated by measuring its effect on the accuracy of a subsequent PR stage that was trained to discriminate between two BMSC cultures of differing quality. The accuracy achieved averaged 93% across four commonly used PR algorithms, corresponding to an overall accuracy gain of 17% compared to non-normalised, unsegmented images.
Citation countsare sourced monthly fromand citation databases.
These databases contain citations from different subsets of available publications and different time periods and thus the citation count from each is usually different. Some works are not in either database and no count is displayed. Scopus includes citations from articles published in 1996 onwards, and Web of Science® generally from 1980 onwards.
Citations counts from theindexing service can be viewed at the linked Google Scholar™ search.
Full-text downloadsdisplays the total number of times this work’s files (e.g., a PDF) have been downloaded from QUT ePrints as well as the number of downloads in the previous 365 days. The count includes downloads for all files if a work has more than one.
|Item Type:||Conference Paper|
|Keywords:||segmentation, phase contrast, bone marrow stromal cells, mesenchymal stem cells, pattern recognition|
|Subjects:||Australian and New Zealand Standard Research Classification > INFORMATION AND COMPUTING SCIENCES (080000) > ARTIFICIAL INTELLIGENCE AND IMAGE PROCESSING (080100)|
|Divisions:||Past > QUT Faculties & Divisions > Faculty of Built Environment and Engineering|
Current > Institutes > Institute of Health and Biomedical Innovation
Past > Schools > School of Engineering Systems
|Copyright Owner:||Copyright 2008 IEEE|
|Deposited On:||11 Dec 2008 08:08|
|Last Modified:||01 Mar 2012 00:12|
Repository Staff Only: item control page