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In vitro characterisation of koala Chlamydia pneumoniae: Morphology, inclusion development and doubling time

Mitchell, Candice M., Mathews, Sarah A., Theodoropoulos, Christina, & Timms, Peter (2008) In vitro characterisation of koala Chlamydia pneumoniae: Morphology, inclusion development and doubling time. Veterinary Microbiology, 1-2, pp. 91-99.

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Abstract

Chlamydia pneumoniae is a common human and animal pathogen associated with upper and lower respiratory tract infections. Of the animal C. pneumoniae isolates, the koala nasal isolate (LPCoLN) is by far the best genetically characterised. This current study was designed to characterise the morphology and developmental events for the LPCoLN isolate, and our results showed several striking in vitro growth differences when compared to the human isolate, AR39. The LPCoLN inclusion size and morphology was distinct from AR39, and a much faster doubling time (3.4–4.9 h versus 5.9–8.7 h doubling time) was observed when grown in HEp-2 cell monolayers. Confocal and electron microscopy of LPCoLN confirmed large (9–30 μm in diameter) inclusions, that were heterogeneously shaped, compared to the small (5–9 μm in diameter), uniformly shaped inclusions of AR39. The morphology of the LPCoLN elementary body was round, and had a narrow or nonexistent periplasmic space, compared to the 'pear-shaped' morphology of AR39 EBs. While both isolates showed evidence of inclusion fusion, the level of fusion was much higher for LPCoLN (100%) compared to AR39 (30–40%). Our findings have provided new insights and identified key differences in the in vitro doubling time, size and morphology of an animal C. pneumoniae isolate.

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8 citations in Scopus
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6 citations in Web of Science®

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ID Code: 17098
Item Type: Journal Article
Additional Information: For more information, please refer to the journal's website (see hypertext link) or contact the author.
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Keywords: Chlamydia pneumoniae, inclusion morphology, characterisation, developmental cycle, elementary body
DOI: 10.1016/j.vetmic.2008.10.008
ISSN: 0378-1135
Subjects: Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > EVOLUTIONARY BIOLOGY (060300) > Evolution of Developmental Systems (060305)
Divisions: Past > QUT Faculties & Divisions > Faculty of Science and Technology
Current > Institutes > Institute of Health and Biomedical Innovation
Past > Schools > School of Life Sciences
Copyright Owner: Copyright 2008 Elsevier
Deposited On: 05 Jan 2009 12:31
Last Modified: 29 Feb 2012 23:49

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