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A minimal common osteochondrocytic differentiation media for the osteogenic and chondrogenic differentiation of bone marrow stromal cells in the construction of osteochondral graft

Li, Jian, Mareddy, Shobha R., Tan, Dawn Meifang, Crawford, Ross W., Long, Xing, Miao, Xigeng, & Xiao, Yin (2009) A minimal common osteochondrocytic differentiation media for the osteogenic and chondrogenic differentiation of bone marrow stromal cells in the construction of osteochondral graft. Tissue Engineering Part A, 15(9), pp. 2481-2490.

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Abstract

To regenerate the complex tissue such as bone-cartilage construct using tissue engineering approach, controllable differentiation of bone marrow stromal cells (BMSCs) into chondrogenic and osteogenic lineages is crucially important. This study proposes to test a minimum common osteochondrocytic differentiation medium (MCDM) formulated by including common soluble supplements (dexamethasone and ascorbic acid) used to induce chondrogenic and osteogenic differentiation. The MCDM coupled with supplemented growth factors was tested for its ability to differentiate BMSCs into osteogenic and chondrogenic lineages in both 2-dimensional (2D) and 3-dimensional (3D) culture systems. When transforming growth factor β3 (TGF-β3) was added to MCDM, BMSCs differentiated to chondrocyte-like cells evidenced by the expression of glycosaminoglycans and type II collagen, whereas osteogenic differentiation was induced by supplementing osteogenic protein-1 (OP-1), resulting in detectable expression of osteopontin and osteocalcin. These chondrogenic and osteogenic differentiation markers were significantly enhanced in the 3D cultures compared to the 2D monolayer cultures. The results achieved in this study lay a foundation for future development of osteochondral graft, which could be engineered from bilayered scaffold with spatially loaded growth factors to control BMSC differentiation.

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10 citations in Scopus
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8 citations in Web of Science®

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ID Code: 17638
Item Type: Journal Article
Additional Information: Self-archiving of the author-version is not yet supported by this publisher. For more information, please refer to the journal's website (see hypertext link) or contact the author.
Keywords: BMSCs, Osteogenic, Chondrogenic, Osteochondral, Differentiation
DOI: 10.1089/ten.tea.2008.0463
ISSN: 1937-3341
Divisions: Past > QUT Faculties & Divisions > Faculty of Built Environment and Engineering
Current > Institutes > Institute of Health and Biomedical Innovation
Past > Schools > School of Engineering Systems
Copyright Owner: Copyright 2009 Mary Ann Liebert
Deposited On: 11 Feb 2009 09:09
Last Modified: 29 Feb 2012 23:54

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