Atomic force microscopy measurements of bovine serum albumin adhesion forces surfaces

Motta, Nunzio, Lai, Chun-Chih, & Bell, John M. (2008) Atomic force microscopy measurements of bovine serum albumin adhesion forces surfaces. International Journal of Nanoscience, 7(6), pp. 299-303.

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A novel, direct technique has been developed to measure the interactions of bovine serum albumin (BSA) on surfaces by using atomic force microscopy (AFM) in a liquid environment. We have been able to measure adhesion forces between proteins and substrate surfaces in phosphatebuffered saline (PBS) solution directly, without any modification to the substrate and the AFM tip. Two different surfaces have been used in the measurements: mica (hydrophilic surface) and polystyrene (hydrophobic surface).The results show that a polystyrene surface has larger adhesion forces to BSA than a mica surface. This is consistent with previous research, which demonstrated that hydrophobic surfaces enhance protein adhesion but hydrophilic surfaces do not.

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ID Code: 19143
Item Type: Journal Article
Refereed: Yes
Keywords: Adhesion, BSA, Proteins, Surface, Atomic Force microscope
DOI: 10.1142/S0219581X08005468
ISSN: 0219-581X
Subjects: Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > BIOCHEMISTRY AND CELL BIOLOGY (060100) > Cellular Interactions (incl. Adhesion Matrix Cell Wall) (060106)
Australian and New Zealand Standard Research Classification > ENGINEERING (090000) > BIOMEDICAL ENGINEERING (090300) > Biomedical Engineering not elsewhere classified (090399)
Australian and New Zealand Standard Research Classification > PHYSICAL SCIENCES (020000) > OTHER PHYSICAL SCIENCES (029900) > Medical Physics (029903)
Divisions: Past > QUT Faculties & Divisions > Faculty of Built Environment and Engineering
Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright 2008 World Scientific Publishing Company
Deposited On: 26 Mar 2009 02:33
Last Modified: 02 Jul 2017 14:40

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