QUT ePrints

Atomic force microscopy measurements of bovine serum albumin adhesion forces surfaces

Motta, Nunzio, Lai, Chun-Chih, & Bell, John M. (2008) Atomic force microscopy measurements of bovine serum albumin adhesion forces surfaces. International Journal of Nanoscience, 7(6), pp. 299-303.

View at publisher

Abstract

A novel, direct technique has been developed to measure the interactions of bovine serum albumin (BSA) on surfaces by using atomic force microscopy (AFM) in a liquid environment. We have been able to measure adhesion forces between proteins and substrate surfaces in phosphatebuffered saline (PBS) solution directly, without any modification to the substrate and the AFM tip. Two different surfaces have been used in the measurements: mica (hydrophilic surface) and polystyrene (hydrophobic surface).The results show that a polystyrene surface has larger adhesion forces to BSA than a mica surface. This is consistent with previous research, which demonstrated that hydrophobic surfaces enhance protein adhesion but hydrophilic surfaces do not.

Impact and interest:

4 citations in Scopus
Search Google Scholar™

Citation countsare sourced monthly from Scopus and Web of Science® citation databases.

These databases contain citations from different subsets of available publications and different time periods and thus the citation count from each is usually different. Some works are not in either database and no count is displayed. Scopus includes citations from articles published in 1996 onwards, and Web of Science® generally from 1980 onwards.

Citations counts from the Google Scholar™ indexing service can be viewed at the linked Google Scholar™ search.

Full-text downloads:

415 since deposited on 26 Mar 2009
37 in the past twelve months

Full-text downloadsdisplays the total number of times this work’s files (e.g., a PDF) have been downloaded from QUT ePrints as well as the number of downloads in the previous 365 days. The count includes downloads for all files if a work has more than one.

ID Code: 19143
Item Type: Journal Article
Keywords: Adhesion, BSA, Proteins, Surface, Atomic Force microscope
DOI: 10.1142/S0219581X08005468
ISSN: 0219-581X
Subjects: Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > BIOCHEMISTRY AND CELL BIOLOGY (060100) > Cellular Interactions (incl. Adhesion Matrix Cell Wall) (060106)
Australian and New Zealand Standard Research Classification > ENGINEERING (090000) > BIOMEDICAL ENGINEERING (090300) > Biomedical Engineering not elsewhere classified (090399)
Australian and New Zealand Standard Research Classification > PHYSICAL SCIENCES (020000) > OTHER PHYSICAL SCIENCES (029900) > Medical Physics (029903)
Divisions: Past > QUT Faculties & Divisions > Faculty of Built Environment and Engineering
Current > Institutes > Institute of Health and Biomedical Innovation
Past > Schools > School of Engineering Systems
Copyright Owner: Copyright 2008 World Scientific Publishing Company
Deposited On: 26 Mar 2009 12:33
Last Modified: 29 Feb 2012 23:47

Export: EndNote | Dublin Core | BibTeX

Repository Staff Only: item control page