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Albumine adhesion force measurements using an atomic force microscopy

Lai, Chun-Chih , Bell, John M., & Motta, Nunzio (2008) Albumine adhesion force measurements using an atomic force microscopy. Advanced Materials Research, 32, pp. 49-52.

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Abstract

A new, direct method has been developed to measure the adhesion forces of bovine serum albumine (BSA) by using Atomic Force Microscopy (AFM) in liquid environment. We were able to measure interactions between proteins and substrate surface in PBS solution directly without any modification to the substrate or the AFM tip. Two different surfaces have been used in the experiments: mica (hydrophilic surface) and polystyrene (hydrophobic surface). The results show that a polystyrene surface is more adhesive to BSA than a mica surface. This is consistent with previous research, which assessed that hydrophobic surfaces enhance protein adhesion but hydrophilic surfaces do not, demonstrating the effectiveness of the technique.

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ID Code: 19632
Item Type: Journal Article
Additional URLs:
Keywords: Atomic Force Microscopy, BSA, Adhesion, Surfaces
DOI: 10.4028/www.scientific.net/AMR.32.49
ISSN: 1022-6680
Subjects: Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > BIOCHEMISTRY AND CELL BIOLOGY (060100) > Cellular Interactions (incl. Adhesion Matrix Cell Wall) (060106)
Australian and New Zealand Standard Research Classification > PHYSICAL SCIENCES (020000) > CONDENSED MATTER PHYSICS (020400) > Surfaces and Structural Properties of Condensed Matter (020406)
Australian and New Zealand Standard Research Classification > TECHNOLOGY (100000) > NANOTECHNOLOGY (100700) > Nanobiotechnology (100703)
Australian and New Zealand Standard Research Classification > TECHNOLOGY (100000) > NANOTECHNOLOGY (100700) > Nanoscale Characterisation (100712)
Divisions: Past > QUT Faculties & Divisions > Faculty of Built Environment and Engineering
Current > Institutes > Institute of Health and Biomedical Innovation
Past > Schools > School of Engineering Systems
Copyright Owner: Copyright 2008 Trans Tech Publications Ltd.
Deposited On: 21 Apr 2009 09:29
Last Modified: 09 Jun 2010 23:35

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