Epitope map for a growth hormone receptor agonist monoclonal antibody, MAb 263

Brown, Richard, Harris, Jonathan, Wan, Yu, Waters, Michael J., & Zheng, Yuan (2003) Epitope map for a growth hormone receptor agonist monoclonal antibody, MAb 263. Molecular Endocrinology, 17(11), pp. 2240-2250.

View at publisher

Abstract

Monoclonal antibody (MAb) 263 is a widely used monoclonal antibody that recognizes the extracellular domain (ECD) of the GH receptor. It has been shown to act as a GH agonist both in vitro and in vivo, and we report here that it must be divalent to exert its effect on the full-length receptor. To understand the mechanism of its agonist action, we have determined the precise epitope for this antibody using a novel random PCR mutagenesis approach together with expression screening in yeast. A library of 5200 clones of rabbit GH receptor ECD mutants were screened both with MAb 263 and with an anticarboxy-tag antibody to verify complete ECD expression. Sequencing for clones that expressed complete ECD but were not MAb 263 positive identified 20 epitope residues distributed in a discontinuous manner throughout the ECD. The major part of the epitope, as revealed after mapping onto the crystal structure model of the ECD molecule, was located on the side and upper portion of domain 1, particularly within the D–E strand disulfide loop 79–96. Molecular dynamics docking of an antibody of the same isotype as MAb 263 was used to dock the bivalent antibody to the 1528-Å2 epitope and to visualize the likely consequences of MAb binding. The minimized model enables the antibody to grasp two receptors in a pincer-like movement from opposite sides, facilitating alignment of the receptor dimerization domains in a manner similar to, but not identical with, GH.

Impact and interest:

25 citations in Scopus
Search Google Scholar™
23 citations in Web of Science®

Citation counts are sourced monthly from Scopus and Web of Science® citation databases.

These databases contain citations from different subsets of available publications and different time periods and thus the citation count from each is usually different. Some works are not in either database and no count is displayed. Scopus includes citations from articles published in 1996 onwards, and Web of Science® generally from 1980 onwards.

Citations counts from the Google Scholar™ indexing service can be viewed at the linked Google Scholar™ search.

ID Code: 22056
Item Type: Journal Article
Refereed: Yes
Additional URLs:
DOI: 10.1210/me.2003-0162
ISSN: 0888-8809
Divisions: Past > QUT Faculties & Divisions > Faculty of Science and Technology
Deposited On: 17 Jun 2009 13:04
Last Modified: 21 Feb 2013 00:46

Export: EndNote | Dublin Core | BibTeX

Repository Staff Only: item control page