Elevated concentration of TNF-a induces trophoblast differentiation in mouse blastocyst outgrowths

Boucaut, Kerry, Garcia-Aragon, Juanita, Harvey, Mark, Herington, Adrian, Teh, Alison, & Whiteside, Eliza (2003) Elevated concentration of TNF-a induces trophoblast differentiation in mouse blastocyst outgrowths. Cell and Tissue Research, 314(2), pp. 275-280.

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Elevated expression of tumour necrosis factora (TNF-a) is associated with adverse pregnancy outcome. This study has examined the expression of TNF-a and its receptors (TNF-Rs) by mouse blastocysts and blastocyst outgrowths from day 4 to 9.5 of pregnancy and investigated the effects of elevated TNF-a on the inner cell mass (ICM) and trophoblast cells of blastocyst outgrowths. RTPCR demonstrated TNF-a mRNA expression from day 7.5 to 9.5, TNF-R1 from day 6.5 to 9.5 and TNF-R2 from day 5.5 to 7.5 of pregnancy, and in situ hybridisation revealed the trophoblast giant cells (TGCs) of the early placenta as the site of TNF-a expression. Day 4 blastocysts were cultured in a physiologically high concentration of TNF-a (100 ng/ml) for 72 h to the outgrowth stage and then compared to blastocysts cultured in media alone. TNF-a-treated blastocyst outgrowths exhibited a significant reduction in ICM cells (mean € SD 23.90€10.42 vs 9.37€7.45, t-test, P<0.0001) with no significant change in the numbers of trophoblast cells (19.97€8.14 vs 21.73€7.79, t-test, P=0.39). Within the trophoblast cell population, the TNF-a-treated outgrowths exhibited a significant increase in multinucleated cells (14.10€5.53 vs 6.37€5.80, t-test, P<0.0001) and a corresponding significant decrease in mononucleated cells (5.87€3.60 vs 15.37€5.87, t-test, P<0.0001). In summary, this study describes the expression of TNF-a and its receptors during the peri-implantation period in the mouse. It also reports that elevated TNF-a restricts ICM proliferation in the blastocyst and changes the ratio of mononucleated to multinucleated trophoblast cells. These findings suggest a mechanism by which increased

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11 citations in Web of Science®
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ID Code: 23264
Item Type: Journal Article
Refereed: Yes
DOI: 10.1007/s00441-003-0791-4
ISSN: 0302-766X
Divisions: Past > QUT Faculties & Divisions > Faculty of Science and Technology
Copyright Owner: Copyright 2003 Springer-Verlag
Copyright Statement:

This is the author-version of the work.

Conference proceedings published, by Springer Verlag, will be available via SpringerLink. http://www.springerlink.com

Deposited On: 17 Jun 2009 13:47
Last Modified: 29 Feb 2012 13:01

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