Polyurethane (PU) scaffolds prepared by solvent casting/particulate leaching (SCPL) combined with centrifugation

Sin, DongChoon, Miao, Xigeng, Liu, Gang, Wei, Fan, Chadwick, Gary, Yan, Cheng, & Friis, Thor (2010) Polyurethane (PU) scaffolds prepared by solvent casting/particulate leaching (SCPL) combined with centrifugation. Materials Science and Engineering : C, 30(1), pp. 78-85.

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This article reports an enhanced solvent casting/particulate (salt) leaching (SCPL) method developed for preparing three-dimensional porous polyurethane (PU) scaffolds for cardiac tissue engineering. The solvent for the preparation of the PU scaffolds was a mixture of dimethylformamide (DFM) and tetrahydrofuran (THF). The enhanced method involved the combination of a conventional SCPL method and a step of centrifugation, with the centrifugation being employed to improve the pore uniformity and the pore interconnectivity of scaffolds. Highly porous three-dimensional scaffolds with a well interconnected porous structure could be achieved at the polymer solution concentration of up to 20% by air or vacuum drying to remove the solvent. When the salt particle sizes of 212-295, 295-425, or 425-531 µm and a 15% w/v polymer solution concentration were used, the porosity of the scaffolds was between 83-92% and the compression moduli of the scaffolds were between 13 kPa and 28 kPa. Type I collagen acidic solution was introduced into the pores of a PU scaffold to coat the collagen onto the pore walls throughout the whole PU scaffold. The human aortic endothelial cells (HAECs) cultured in the collagen-coated PU scaffold for 2 weeks were observed by scanning electron microscopy (SEM). It was shown that the enhanced SCPL method and the collagen coating resulted in a spatially uniform distribution of cells throughout the collagen-coated PU scaffold.

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47 citations in Web of Science®
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ID Code: 27181
Item Type: Journal Article
Refereed: Yes
Additional URLs:
Keywords: Polyurethane scaffolds, salt leaching, centrifugation, pore interconnectivity, human aortic endothelial cells, collagen coating
DOI: 10.1016/j.msec.2009.09.002
ISSN: 0928-4931
Divisions: Past > QUT Faculties & Divisions > Faculty of Built Environment and Engineering
Current > Institutes > Institute of Health and Biomedical Innovation
Past > Schools > School of Engineering Systems
Copyright Owner: Copyright 2009 Elsevier B.V.
Copyright Statement: This is the author’s version of a work that was accepted for publication in Materials Science and Engineering : C. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Materials Science and Engineering : C, [VOL 30, ISSUE 1, (2010)] DOI: 10.1016/j.msec.2009.09.002
Deposited On: 02 Sep 2009 22:16
Last Modified: 22 Mar 2016 19:38

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