In vivo confocal microscopy of the palpebral conjunctiva and tarsal plate

Efron, Nathan, Al-Dossari, Munira, & Pritchard, Nicola (2009) In vivo confocal microscopy of the palpebral conjunctiva and tarsal plate. Optometry and Vision Science, 86(11), pp. 1303-1308.

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Purpose The aim of this work is to develop a more complete understanding of the in vivo histology of the human palpebral conjunctiva and tarsal plate. Methods. The upper eyelids of 11 healthy human volunteer subjects were everted, and laser scanning confocal microscopy was used to examine the various tissue layers of the palpebral conjunctiva and tarsal plate.

Results The superficial and basal epithelial layers are composed of cells with gray cytoplasm and thick, light gray borders.Nuclei can not be seen. The stroma has a varied appearance; fibrous tissue is sometimes observed, interspersed with dark,amorphous lacunae, and crevases. Numerous single white or gray cells populate this tissue, and fine blood vessels are seen traversing the field. Occasional conjunctival microcysts and Langerhans cells are observed. The tarsal plate is dark and amorphous, and meibomian gland acini with convoluted borders are clearly observed. Acini are composed of an outer lining of large cuboidal cells, and differentiated secretory cells can be seen within the acini lumen.

Conclusions Laser scanning confocal microscopy is capable of studying the human palpebral conjunctiva, tarsal plate, and acini of meibomian glands in vivo. The observations presented here may provide useful supplementary anatomical information relating to the morphology of this tissue.

Impact and interest:

23 citations in Scopus
16 citations in Web of Science®
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ID Code: 28978
Item Type: Journal Article
Refereed: Yes
Additional Information: This article can be accessed free of charge from the journal website 12 months after the publication date. See Official URL above.
Keywords: palpebral conjunctiva, Laser Scanning confocal microscopy, epithelium, conjuctival microcysts, tarsal plate, meibomian gland, langerhans cells, OAVJ
DOI: 10.1097/OPX.0b013e3181bc652e
ISSN: 1040-5488
Subjects: Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > OPTOMETRY AND OPHTHALMOLOGY (111300) > Optometry and Ophthalmology not elsewhere classified (111399)
Divisions: Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Current > Schools > School of Optometry & Vision Science
Copyright Owner: Copyright 2009 Elsevier for the American Academy of Optometry
Deposited On: 08 Jan 2010 02:24
Last Modified: 09 Feb 2015 04:12

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