Simultaneous determination of three synthetic glucocorticoids by differential pulse stripping voltammetry with the aid of chemometrics

Ni, Yongnian, Li, Shuzhen, & Kokot, Serge (2008) Simultaneous determination of three synthetic glucocorticoids by differential pulse stripping voltammetry with the aid of chemometrics. Analytical Letters, 41(11), pp. 2058-2076.

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Abstract

A novel voltammetric method for simultaneous determination of the glucocorticoid residues prednisone, prednisolone, and dexamethasone was developed. All three compounds were reduced at a mercury electrode in a Britton-Robinson buffer (pH 3.78), and well-defined voltammetric waves were observed. However, the voltammograms of these three compounds overlapped seriously and showed nonlinear character, and thus, it was difficult to analyze the compounds individually in their mixtures. In this work, two chemometrics methods, principal component regression (PCR) and partial least squares (PLS), were applied to resolve the overlapped voltammograms, and the calibration models were established for simultaneous determination of these compounds. Under the optimum experimental conditions, the limits of detection (LOD) were 5.6, 8.3, and 16.8 µg l-1 for prednisone, prednisolone, and dexamethasone, respectively. The proposed method was also applied for the determination of these glucocorticoid residues in the rabbit plasma and human urine samples with satisfactory results.

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2 citations in Web of Science®

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ID Code: 29038
Item Type: Journal Article
Refereed: Yes
Keywords: Chemometrics, Differential Pulse Stripping Voltammetry, Glucocorticoid Residues, Rabbit Plasma, Urine
DOI: 10.1080/00032710802208708
ISSN: 0003-2719
Divisions: Past > QUT Faculties & Divisions > Faculty of Science and Technology
Past > Schools > School of Physical & Chemical Sciences
Deposited On: 03 Dec 2009 05:59
Last Modified: 29 Feb 2012 13:43

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