NMR measurement of small-molecule diffusionin PVA hydrogels: a comparison of CONVEX and standard PGSE methods
Regan, David G., Momot, Konstantin I., Martens, Penny J., Kuchel, Philip W., & Poole-Warren, Laura A. (2006) NMR measurement of small-molecule diffusionin PVA hydrogels: a comparison of CONVEX and standard PGSE methods. Diffusion Fundamentals Online, 4, 1.1-1.18.
Hydrogels are biocompatible polymeric materials that are becoming increasingly important in biomedical applications, such as drug delivery and tissue engineering. Understanding of small-molecule diffusion in these systems is important in the contexts of controlled drug release; transport of nutrients (e.g., O2 and growth factors) into the gel; and transport of cellular waste out of the gel. In this work, the diffusion coefficient of the aromatic amino acid phenylalanine (Phe) in non-crosslinked and crosslinked poly(vinyl alcohol) (PVA) hydrogels was measured using two NMR diffusion methods, CONVEX and the standard pulsed-gradient spin-echo (PGSE). Pulsed field-gradient (PFG) NMR measurements provide the advantage of measuring the molecular selfdiffusion coefficient directly and without having to rely on the physical release of the solute, but are often difficult to perform in tissues and hydrated polymers due to a large water signal. CONVEX is a recently proposed diffusion method that alleviates this problem by means of NMR excitation-sculpting water suppression. In the measurements presented here, CONVEX results were superior to those from PGSE measurements with respect to every test applied, and enabled a reliable comparison of the diffusion coefficients of Phe in crosslinked and non-crosslinked hydrogels. The value of D(Phe) was smaller in the non-crosslinked hydrogel than in the crosslinked gel; this finding is discussed in the paper.
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|Item Type:||Journal Article|
|Keywords:||NMR solvent suppression, phenylalanine, poly(vinyl alcohol), Stejskal, Tanner plot, diffusion in a polymer network, metabolite transport in tissue scaffolds|
|Subjects:||Australian and New Zealand Standard Research Classification > CHEMICAL SCIENCE (030000)|
|Divisions:||Past > QUT Faculties & Divisions > Faculty of Science and Technology|
|Copyright Owner:||Copyright 2006 (please consult author)|
|Copyright Statement:||The contents of this journal can be freely accessed online via the journal’s web page (see link).|
|Deposited On:||14 Dec 2005 00:00|
|Last Modified:||29 Feb 2012 13:25|
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