QUT ePrints

Comparison of methods for processing drinking water samples for the isolation of Myocbacterium avium and intracellulare

Thomson, Rachel, Carter, Robyn, Gilpin, Chris, Coulter, Chris, & Hargreaves, Megan (2008) Comparison of methods for processing drinking water samples for the isolation of Myocbacterium avium and intracellulare. Applied and Environmental Microbiology, 74(10).

View at publisher

Abstract

Several protocols for isolation of mycobacteria from water exist, but there is no established standard method. This study compared methods of processing potable water samples for the isolation of Mycobacterium avium and Mycobacterium intracellulare using spiked sterilized water and tap water decontaminated using 0.005% cetylpyridinium chloride (CPC). Samples were concentrated by centrifugation or filtration and inoculated onto Middlebrook 7H10 and 7H11 plates and Lowenstein-Jensen slants and into mycobacterial growth indicator tubes with or without polymyxin, azlocillin, nalidixic acid, trimethoprim, and amphotericin B. The solid media were incubated at 32°C, at 35°C, and at 35°C with CO2 and read weekly. The results suggest that filtration of water for the isolation of mycobacteria is a more sensitive method for concentration than centrifugation. The addition of sodium thiosulfate may not be necessary and may reduce the yield. Middlebrook M7H10 and 7H11 were equally sensitive culture media. CPC decontamination, while effective for reducing growth of contaminants, also significantly reduces mycobacterial numbers. There was no difference at 3 weeks between the different incubation temperatures.

Impact and interest:

13 citations in Scopus
Search Google Scholar™
13 citations in Web of Science®

Citation countsare sourced monthly from Scopus and Web of Science® citation databases.

These databases contain citations from different subsets of available publications and different time periods and thus the citation count from each is usually different. Some works are not in either database and no count is displayed. Scopus includes citations from articles published in 1996 onwards, and Web of Science® generally from 1980 onwards.

Citations counts from the Google Scholar™ indexing service can be viewed at the linked Google Scholar™ search.

Full-text downloads:

114 since deposited on 09 Dec 2009
24 in the past twelve months

Full-text downloadsdisplays the total number of times this work’s files (e.g., a PDF) have been downloaded from QUT ePrints as well as the number of downloads in the previous 365 days. The count includes downloads for all files if a work has more than one.

ID Code: 29209
Item Type: Journal Article
Keywords: mycobacteria, drinking water
DOI: 10.1128/AEM.02009-07
ISSN: 0099-2240
Subjects: Australian and New Zealand Standard Research Classification > ENVIRONMENTAL SCIENCES (050000) > OTHER ENVIRONMENTAL SCIENCES (059900) > Environmental Sciences not elsewhere classified (059999)
Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > PUBLIC HEALTH AND HEALTH SERVICES (111700) > Environmental and Occupational Health and Safety (111705)
Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > PUBLIC HEALTH AND HEALTH SERVICES (111700) > Public Health and Health Services not elsewhere classified (111799)
Divisions: Past > QUT Faculties & Divisions > Faculty of Science and Technology
Current > Institutes > Institute of Health and Biomedical Innovation
Past > Schools > School of Life Sciences
Copyright Owner: Copyright 2008 American Society for Microbiology. All Rights Reserved.
Deposited On: 10 Dec 2009 09:52
Last Modified: 29 Feb 2012 23:53

Export: EndNote | Dublin Core | BibTeX

Repository Staff Only: item control page