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Prevalence and occurrence of zoonotic bacterial pathogens in surface waters determined by quantitative PCR

Ahmed, Warish, Sawant, Shewata, Huygens, Flavia, Goonetilleke, Ashantha, & Gardner, Ted (2009) Prevalence and occurrence of zoonotic bacterial pathogens in surface waters determined by quantitative PCR. Water Research, 43(19), pp. 4918-4928.

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Abstract

The prevalence and concentrations of Campylobacter jejuni, Salmonella spp. and enterohaemorrhagic E. coli (EHEC) were investigated in surface waters in Brisbane, Australia using quantitative PCR (qPCR) based methodologies. Water samples were collected from Brisbane City Botanic Gardens (CBG) Pond, and two urban tidal creeks (i.e., Oxley Creek and Blunder Creek). Of the 32 water samples collected, 8 (25%), 1 (3%), 9 (28%), 14 (44%), and 15 (47%) were positive for C. jejuni mapA, Salmonella invA, EHEC O157 LPS, EHEC VT1, and EHEC VT2 genes, respectively. The presence/absence of the potential pathogens did not correlate with either E. coli or enterococci concentrations as determined by binary logistic regression. In conclusion, the high prevalence, and concentrations of potential zoonotic pathogens along with the concentrations of one or more fecal indicators in surface water samples indicate a poor level of microbial quality of surface water, and could represent a significant health risk to users. The results from the current study would provide valuable information to the water quality managers in terms of minimizing the risk from pathogens in surface waters.

Impact and interest:

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18 citations in Web of Science®

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ID Code: 29216
Item Type: Journal Article
Keywords: Surface water, Fecal pollution, Zoonotic pathogens, Fecal indicators, Health risk
DOI: 10.1016/j.watres.2009.03.041
ISSN: 0043-1354
Subjects: Australian and New Zealand Standard Research Classification > ENGINEERING (090000) > ENVIRONMENTAL ENGINEERING (090700) > Environmental Engineering Modelling (090702)
Australian and New Zealand Standard Research Classification > ENVIRONMENTAL SCIENCES (050000) > ENVIRONMENTAL SCIENCE AND MANAGEMENT (050200) > Environmental Monitoring (050206)
Divisions: Past > QUT Faculties & Divisions > Faculty of Built Environment and Engineering
Past > QUT Faculties & Divisions > Faculty of Science and Technology
Past > Schools > School of Urban Development
Copyright Owner: Copyright 2009 Published by Elsevier Ltd.
Deposited On: 10 Dec 2009 11:04
Last Modified: 29 Feb 2012 23:54

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