Evaluation of multiple sewage-associated bacteroides PCR markers for sewage pollution tracking

Ahmed, Warish, Goonetilleke, Ashantha, Powell, Daniel, & Gardner, Ted (2009) Evaluation of multiple sewage-associated bacteroides PCR markers for sewage pollution tracking. Water Research, 43(19), pp. 4872-4877.

View at publisher


The host specificity of the five published sewage-associated Bacteroides markers (i.e., HF183, BacHum, HuBac, BacH and Human-Bac) was evaluated in Southeast Queensland, Australia by testing fecal DNA samples (n = 186) from 11 animal species including human fecal samples collected via influent to a sewage treatment plant (STP). All human fecal samples (n = 50) were positive for all five markers indicating 100% sensitivity of these markers. The overall specificity of the HF183 markers to differentiate between humans and animals was 99%. The specificities of the BacHum and BacH markers were > 94%, suggesting that these markers are suitable for sewage pollution in environmental waters in Australia. The BacHum (i.e., 63% specificity) and Human-Bac (i.e., 79% specificity) markers performed poorly in distinguishing between the sources of human and animal fecal samples. It is recommended that the specificity of the sewage-associated markers must be rigorously tested prior to its application to identify the sources of fecal pollution in environmental waters.

Impact and interest:

36 citations in Scopus
Search Google Scholar™
35 citations in Web of Science®

Citation counts are sourced monthly from Scopus and Web of Science® citation databases.

These databases contain citations from different subsets of available publications and different time periods and thus the citation count from each is usually different. Some works are not in either database and no count is displayed. Scopus includes citations from articles published in 1996 onwards, and Web of Science® generally from 1980 onwards.

Citations counts from the Google Scholar™ indexing service can be viewed at the linked Google Scholar™ search.

Full-text downloads:

474 since deposited on 09 Dec 2009
19 in the past twelve months

Full-text downloads displays the total number of times this work’s files (e.g., a PDF) have been downloaded from QUT ePrints as well as the number of downloads in the previous 365 days. The count includes downloads for all files if a work has more than one.

ID Code: 29217
Item Type: Journal Article
Refereed: Yes
Additional URLs:
Keywords: Fecal pollution, Water quality, Microbial source tracking, Bacteroides, Sewage-associated markers
DOI: 10.1016/j.watres.2009.08.042
ISSN: 0043-1354
Subjects: Australian and New Zealand Standard Research Classification > ENGINEERING (090000) > ENVIRONMENTAL ENGINEERING (090700) > Environmental Engineering Modelling (090702)
Australian and New Zealand Standard Research Classification > ENVIRONMENTAL SCIENCES (050000) > ENVIRONMENTAL SCIENCE AND MANAGEMENT (050200) > Environmental Monitoring (050206)
Divisions: Past > QUT Faculties & Divisions > Faculty of Built Environment and Engineering
Past > Schools > School of Urban Development
Copyright Owner: Copyright 2009 Elsevier
Deposited On: 09 Dec 2009 23:28
Last Modified: 29 Feb 2012 13:57

Export: EndNote | Dublin Core | BibTeX

Repository Staff Only: item control page