Evaluation of multiple sewage-associated bacteroides PCR markers for sewage pollution tracking
Ahmed, Warish, Goonetilleke, Ashantha, Powell, Daniel, & Gardner, Ted (2009) Evaluation of multiple sewage-associated bacteroides PCR markers for sewage pollution tracking. Water Research, 43(19), pp. 4872-4877.
The host specificity of the five published sewage-associated Bacteroides markers (i.e., HF183, BacHum, HuBac, BacH and Human-Bac) was evaluated in Southeast Queensland, Australia by testing fecal DNA samples (n = 186) from 11 animal species including human fecal samples collected via influent to a sewage treatment plant (STP). All human fecal samples (n = 50) were positive for all five markers indicating 100% sensitivity of these markers. The overall specificity of the HF183 markers to differentiate between humans and animals was 99%. The specificities of the BacHum and BacH markers were > 94%, suggesting that these markers are suitable for sewage pollution in environmental waters in Australia. The BacHum (i.e., 63% specificity) and Human-Bac (i.e., 79% specificity) markers performed poorly in distinguishing between the sources of human and animal fecal samples. It is recommended that the specificity of the sewage-associated markers must be rigorously tested prior to its application to identify the sources of fecal pollution in environmental waters.
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|Item Type:||Journal Article|
|Keywords:||Fecal pollution, Water quality, Microbial source tracking, Bacteroides, Sewage-associated markers|
|Subjects:||Australian and New Zealand Standard Research Classification > ENGINEERING (090000) > ENVIRONMENTAL ENGINEERING (090700) > Environmental Engineering Modelling (090702)|
Australian and New Zealand Standard Research Classification > ENVIRONMENTAL SCIENCES (050000) > ENVIRONMENTAL SCIENCE AND MANAGEMENT (050200) > Environmental Monitoring (050206)
|Divisions:||Past > QUT Faculties & Divisions > Faculty of Built Environment and Engineering|
Past > Schools > School of Urban Development
|Copyright Owner:||Copyright 2009 Elsevier|
|Deposited On:||10 Dec 2009 09:28|
|Last Modified:||29 Feb 2012 23:57|
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