Initiation of embryogenic cell suspensions of taro (Colocasia esculenta var. esculenta) and plant regeneration
Deo, Pradeep C., Taylor, Mary, Tyagi, Anand P., Harding, Robert M., & Becker, Douglas K. (2009) Initiation of embryogenic cell suspensions of taro (Colocasia esculenta var. esculenta) and plant regeneration. Plant Cell, Tissue and Organ Culture, 100(3), pp. 283-291.
Embryogenic callus was initiated by culturing in vitro taro corm slices on agar-solidified half-strength MS medium containing 2.0 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) for 20 days followed by transfer to 1.0 mg/L thidiazuron (TDZ). Callus was subsequently proliferated on solid medium containing 1.0 mg/L TDZ, 0.5 mg/L 2,4- D and 800 mg/L glutamine before transfer to liquid medium containing the same components but with reduced glutamine (100 mg/L). After 3 months in liquid culture on an orbital shaker, cytoplasmically dense cell aggregates began to form. Somatic embryogenesis was induced by plating suspension cells onto solid media containing reduced levels of hormones (0.1 mg/L TDZ, 0.05 mg/L 2,4-D), high concentrations of sucrose (40–50 g/L) and biotin (1.0 mg/L). Embryo maturation and germination was then induced on media containing 0.05 mg/L benzyladenine (BA) and 0.1 mg/L indole-3-acetic acid (IAA). Histological studies of the developing embryos revealed the presence of typical shoot and root poles suggesting that these structures were true somatic embryos. The rate of somatic embryos formation was 500–3,000 per mL settledcell volume while approximately 60% of the embryos regenerated into plants.
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|Item Type:||Journal Article|
|Keywords:||Somatic embryogenesis, Callus, Cell suspension, Colocasia esculenta var. esculenta|
|Subjects:||Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > PLANT BIOLOGY (060700) > Plant Developmental and Reproductive Biology (060703)|
|Divisions:||Current > Research Centres > Centre for Tropical Crops and Biocommodities|
Past > QUT Faculties & Divisions > Faculty of Science and Technology
|Copyright Owner:||Copyright 2009 Springer Science+Business Media B.V.|
|Copyright Statement:||The original publication is available at SpringerLink http://www.springerlink.com|
|Deposited On:||15 Dec 2009 10:40|
|Last Modified:||01 Mar 2012 00:04|
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