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Kinematics of intracellular chlamydiae provide evidence for contact-dependent development

Wilson, D. P., Whittum-Hudson, J. A., Timms, P., & Bavoil, P. M. (2009) Kinematics of intracellular chlamydiae provide evidence for contact-dependent development. Journal of Bacteriology, 191(18), pp. 5734-5742.

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Abstract

A crucial process of chlamydial development involves differentiation of the replicative reticulate body (RB) into the infectious elementary body (EB). We present experimental evidence to provide support for a contact-dependent hypothesis for explaining the trigger involved in differentiation. We recorded live-imaging of Chlamydia trachomatis-infected McCoy cells at key times during development and tracked the temporospatial trajectories of individual chlamydial particles. We found that movement of the particles is related to development. Early to mid-developmental stages involved slight wobbling of RBs. The average speed of particles increased sharply at 24 h postinfection (after the estimated onset of RB to EB differentiation). We also investigated a penicillin-supplemented culture containing EBs, RBs, and aberrantly enlarged, stressed chlamydiae. Near-immobile enlarged particles are consistent with their continued tethering to the chlamydial inclusion membrane (CIM). We found a significantly negative, nonlinear association between speed and size/type of particles, providing further support for the hypothesis that particles become untethered near the onset of RB to EB differentiation. This study establishes the relationship between the motion properties of the chlamydiae and developmental stages, whereby wobbling RBs gradually lose contact with the CIM, and RB detachment from the CIM is coincidental with the onset of late differentiation.

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6 citations in Scopus
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5 citations in Web of Science®

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ID Code: 30261
Item Type: Journal Article
Keywords: Chlamydia, Development, Cycle
DOI: 10.1128/JB.00293-09
ISSN: 0021-9193
Subjects: Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > MICROBIOLOGY (060500) > Bacteriology (060501)
Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > MEDICAL MICROBIOLOGY (110800) > Medical Bacteriology (110801)
Divisions: Past > QUT Faculties & Divisions > Faculty of Science and Technology
Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright 2009 American Society for Microbiology. All Rights Reserved.
Deposited On: 09 Feb 2010 16:31
Last Modified: 29 Feb 2012 23:58

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