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In vivo confocal microscopy of the bulbar conjunctiva

Efron, Nathan, Al-Dossari, Munira, & Pritchard, Nicola (2009) In vivo confocal microscopy of the bulbar conjunctiva. Clinical And Experimental Ophthalmology, 37(4), pp. 335-344.

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Abstract

Background: The aim of this work is to develop a more complete qualitative and quantitative understanding of the in vivo histology of the human bulbar conjunctiva. Methods: Laser scanning confocal microscopy (LSCM) was used to observe and measure morphological characteristics of the bulbar conjunctiva of 11 healthy human volunteer subjects. Results: The superficial epithelial layer of the bulbar conjunctiva is seen as a mass of small cell nuclei. Cell borders are sometimes visible. The light grey borders of basal epithelial cells are clearly visible, but nuclei can not be seen. The conjunctival stroma is comprised of a dense meshwork of white fibres, through which traverse blood vessels containing cellular elements. Orifices at the epithelial surface may represent goblet cells that have opened and expelled their contents. Goblet cells are also observed in the deeper epithelial layers, as well as conjunctival microcysts and mature forms of Langerhans cells. The bulbar conjunctiva has a mean thickness of 32.9 1.1 mm, and a superficial and basal epithelial cell density of 2212 782 and 2368 741 cells/ mm2, respectively. Overall goblet and mature Langerhans cell densities are 111 58 and 23 25 cells/mm2, respectively. Conclusions: LSCM is a powerful technique for studying the human bulbar conjunctiva in vivo and quantifying key aspects of cell morphology. The observations presented here may serve as a useful marker against which changes in conjunctival morphology due to disease, surgery, drug therapy or contact lens wear can be assessed.

Impact and interest:

24 citations in Scopus
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20 citations in Web of Science®

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ID Code: 30354
Item Type: Journal Article
Keywords: Conjunctiva, Goblet cell density, Langerhans, cell density, Laser scanning confocal microscopy
DOI: 10.1111/j.1442-9071.2009.02065.x
Divisions: Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Current > Schools > School of Optometry & Vision Science
Copyright Owner: Copyright 2009 Blackwell Publishing
Deposited On: 11 Feb 2010 12:10
Last Modified: 01 Mar 2012 00:08

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