Use of Tear Enzyme Activities to Assess the Corneal Response to Contact Lens Wear

Fullard, Roderick J. & Carney, Leo G. (1986) Use of Tear Enzyme Activities to Assess the Corneal Response to Contact Lens Wear. Acta Ophthalmologica Scandinavica, 64(2), pp. 216-220.


A non-invasive biochemical method for assessing the effects of contact lens wear on the in vivo corneal epithelium is described. A fluorometric technique is used to measure the activities of lactate dehydrogenase (LDH) and malate dehydrogenase (MDH) in human tear fluid. In view of the corneal epithelial origin of these enzymes, changes in their activity can indicate the severity of environmental stresses on the corneal epithelium. Following short-term wear of contact lenses, LDH and MDH activities are altered so that the tear LDH/MDH ratio is elevated. The magnitude and time course of this elevation are influenced by contact lens type, fit and duration of wear. The technique can yield more specific measures of the corneal response to contact lens wear than previous techniques.

Impact and interest:

9 citations in Scopus
9 citations in Web of Science®
Search Google Scholar™

Citation counts are sourced monthly from Scopus and Web of Science® citation databases.

These databases contain citations from different subsets of available publications and different time periods and thus the citation count from each is usually different. Some works are not in either database and no count is displayed. Scopus includes citations from articles published in 1996 onwards, and Web of Science® generally from 1980 onwards.

Citations counts from the Google Scholar™ indexing service can be viewed at the linked Google Scholar™ search.

ID Code: 3093
Item Type: Journal Article
Refereed: Yes
Additional Information: For more information, please refer to the journal’s website (see link) or contact the author. Author contact details:
Additional URLs:
Keywords: Contact Lenses, Hydrophilic/, adverse effects, L, Lactate Dehydrogenase/, metabolism, Malate Dehydrogenase/, metabolism, Tears/, enzymology, Circadian Rhythm, Comparative Study, Humans, Time Factors
ISSN: 1395-3907
Subjects: Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > OPTOMETRY AND OPHTHALMOLOGY (111300)
Divisions: Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright 1986 Blackwell Publishing
Copyright Statement: The definitive version is available at
Deposited On: 19 Jan 2006 00:00
Last Modified: 15 Jan 2009 06:54

Export: EndNote | Dublin Core | BibTeX

Repository Staff Only: item control page