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Runx2 overexpression in bone marrow stromal cells accelerates bone formation in critically-sized femoral defects

Wojtowicz, Abigail M., Templeman, Kellie L., Hutmacher, Dietmar W., Guldberg, Robert E., & García, Andrés J. (2010) Runx2 overexpression in bone marrow stromal cells accelerates bone formation in critically-sized femoral defects. Tissue Engineering Part A.

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Abstract

The repair of large non-unions in long bones remains a significant clinical problem due to high failure rates and limited tissue availability for auto- and allografts. Many cell-based strategies for healing bone defects deliver bone marrow stromal cells to the defect site to take advantage of the inherent osteogenic capacity of this cell type. However, many factors, including donor age and ex vivo expansion of the cells, cause bone marrow stromal cells to lose their differentiation ability. To overcome these limitations, we have genetically engineered bone marrow stromal cells to constitutively overexpress the osteoblast specific transcription factor Runx2. In the present study, we examined Runx2-modified bone marrow stromal cells, delivered via poly(caprolactone) scaffolds loaded with type I collagen meshes, in critically-sized segmental defects in rats compared to unmodified cells, cell-free scaffolds and empty defects. Runx2 expression in bone marrow stromal cells accelerated healing of critically-sized defects compared to unmodified bone marrow stromal cells and defects receiving cell-free treatments. These findings provide an accelerated method for healing large bone defects which may reduce recovery time and the need for external fixation of critically-sized defects.

Impact and interest:

8 citations in Scopus
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6 citations in Web of Science®

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ID Code: 31433
Item Type: Journal Article
Additional URLs:
DOI: 10.1089/ten.TEA.2010.0025
ISSN: 1076-3279
Subjects: Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > BIOCHEMISTRY AND CELL BIOLOGY (060100) > Biochemistry and Cell Biology not elsewhere classified (060199)
Australian and New Zealand Standard Research Classification > ENGINEERING (090000) > BIOMEDICAL ENGINEERING (090300) > Biomaterials (090301)
Divisions: Past > QUT Faculties & Divisions > Faculty of Built Environment and Engineering
Current > Institutes > Institute of Health and Biomedical Innovation
Past > Schools > School of Engineering Systems
Copyright Owner: Copyright 2010 Mary Ann Liebert Inc.
Deposited On: 19 May 2010 07:41
Last Modified: 01 Mar 2012 00:20

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