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Engineering tubular bone constructs

Chen, Fulin, Zhou, Yefang, Saey Tuan, Barnabas, Woodruff, Maria A., & Hutmacher, Dietmar W. (2007) Engineering tubular bone constructs. Journal of Biomechanics, 40(Supplementary 1), s73-s79.

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Abstract

Cell-sheet techniques have been proven effective in various soft tissue engineering applications. In this experiment, we investigated the feasibility of bone tissue engineering using a hybrid of mesenchymal stem cell (MSC) sheets and PLGA meshes. Porcine MSCs were cultured to a thin layer of cell sheets via osteogenic induction. Tube-like long bones were constructed by wrapping the cell sheet on to PLGA meshes resulting in constructs which could be cultured in spinner flasks, prior to implantation in nude rats. Our results showed that the sheets were composed of viable cells and dense matrix with a thickness of about 80–120 mm, mineral deposition was also observed in the sheet. In vitro cultures demonstrated calcified cartilage-like tissue formation and most PLGA meshes were absorbed during the 8-week culture period. In vivo experiments revealed that dense mineralized tissue was formed in subcutaneous sites and the 8- week plants shared similar micro-CT characteristics with native bone. The neo tissue demonstrated histological markers for both bone and cartilage, indicating that the bone formation pathway in constructs was akin to endochondral ossification, with the residues of PLGA having an effect on the neo tissue organization and formation. These results indicate that cell-sheet approaches in combination with custom-shaped scaffolds have potential in producing bone tissue.

Impact and interest:

15 citations in Scopus
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12 citations in Web of Science®

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ID Code: 32413
Item Type: Journal Article
Additional URLs:
Keywords: bone, scaffold
DOI: 10.1016/j.jbiomech.2007.02.017
ISSN: 0021-9290
Subjects: Australian and New Zealand Standard Research Classification > ENGINEERING (090000) > BIOMEDICAL ENGINEERING (090300) > Biomaterials (090301)
Divisions: Past > QUT Faculties & Divisions > Faculty of Built Environment and Engineering
Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright 2007 Elsevier
Deposited On: 28 May 2010 08:50
Last Modified: 29 Feb 2012 23:57

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