Direct in vivo measurement of corneal epithelial metabolic activity using a polarographic oxygen sensor
Holden, Brien A. , Sulonen, Jukka, Vannas, Antti, Sweeney, Deborah F. , & Efron, Nathan (1985) Direct in vivo measurement of corneal epithelial metabolic activity using a polarographic oxygen sensor. Ophthalmic research, 17(3), pp. 168-173.
The component oxygen fluxes that constitute a single recording of corneal oxygen uptake when a polarographic oxygen sensor (POS) is placed in contact with the cornea were determined in the in vivo rabbit eye by removing the epithelium and injecting an air bubble into the anterior chamber. The relative contribution of epithelial oxygen consumption, stromal oxygen consumption and diffusion were approximately 55, 5 and 40%, respectively. It is demonstrated that differences in corneal oxygen uptake rates recorded with a POS primarily indicate differences in epithelial metabolic activity. Changes in epithelial metabolism can be determined by measuring both corneal oxygen uptake and corneal thickness. For example, if corneal thickness is constant, differences in epithelial oxygen flux (a) between two eyes of one person, or (b) before and after a procedure in the same eye, can be calculated by multiplying the difference in oxygen consumption measured with a POS by a factor of 1.8.
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|Item Type:||Journal Article|
|Additional Information:||For more information, please refer to the journal's website (see link) or contact the author. Author contact details: email@example.com|
|Keywords:||Oxygen Consumption, Cornea/, metabolism, Polarography/, instrumentation, Animals, Epithelium/metabolism, Mathematics, Rabbits, Research Support, Non, U, S, Gov't|
|Subjects:||Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > OPTOMETRY AND OPHTHALMOLOGY (111300)|
|Divisions:||Current > QUT Faculties and Divisions > Faculty of Health|
Current > Institutes > Institute of Health and Biomedical Innovation
|Copyright Owner:||Copyright 1985 Karger|
|Deposited On:||08 Feb 2006|
|Last Modified:||11 Aug 2011 03:43|
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