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Evaluating sewage associated JCV and BKV polyomaviruses for sourcing human fecal pollution in a coastal river in Southeast Queensland Australia

Ahmed, Warish, Wang, Charles, Goonetilleke, Ashantha, & Gardner, Ted (2010) Evaluating sewage associated JCV and BKV polyomaviruses for sourcing human fecal pollution in a coastal river in Southeast Queensland Australia. Journal of Environmental Quality, 39(5), pp. 1743-1750.

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    Abstract

    In this study, the host-sensitivity and -specificity of JCV and BKV polyomaviruses were evaluated by testing wastewater/fecal samples from nine host groups in Southeast Queensland, Australia. The JCV and BKV polyomaviruses were detected in 48 human wastewater samples collected from the primary and secondary effluent suggesting high sensitivity of these viruses in human wastewater. Of the 81 animal wastewater/fecal samples tested, 80 were PCR negative for this marker. Only one sample from pig wastewater was positive. Nonetheless, the overall host-specificity of these viruses to differentiate between human and animal wastewater/fecal samples was 0.99. To our knowledge, this is the first study in Australia that reports the high specificity of JCV and BKV polyomaviruses. To evaluate the field application of these viruses to detect human fecal pollution, 20 environmental samples were collected from a coastal river. Of the 20 samples tested, 15% and 70% samples exceeded the regulatory guidelines for E. coli and enterococci levels for marine waters. In all, 5 (25%) samples were PCR positive for JCV and BKV indicated the presence of human fecal pollution in the studied river. The results suggest that JCV and BKV detection using PCR could be a useful tool for the identification of human sourced fecal pollution in coastal waters.

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    15 citations in Scopus
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    15 citations in Web of Science®

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    ID Code: 34302
    Item Type: Journal Article
    Keywords: Microbial source tracking, River pollution, Fecal pollution, Microbial pollution
    DOI: 10.2134/jeq2010.0062
    Subjects: Australian and New Zealand Standard Research Classification > ENVIRONMENTAL SCIENCES (050000) > OTHER ENVIRONMENTAL SCIENCES (059900)
    Australian and New Zealand Standard Research Classification > ENGINEERING (090000) > ENVIRONMENTAL ENGINEERING (090700) > Environmental Engineering Modelling (090702)
    Divisions: Past > QUT Faculties & Divisions > Faculty of Built Environment and Engineering
    Past > Schools > School of Urban Development
    Copyright Owner: Copyright 2010 by the American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America.
    Deposited On: 02 Sep 2010 09:28
    Last Modified: 21 May 2012 08:28

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