Receptor-DNA interactions: EMSA and footprinting

Read, Jason T., Cheng, Helen, Hendy, Stephen C., Nelson, Colleen C., & Rennie, Paul S. (2009) Receptor-DNA interactions: EMSA and footprinting. In McEwan, Iain J. (Ed.) The Nuclear Receptor Superfamily : Methods and Protocols. Humana, New York, N.Y, pp. 97-122.

View at publisher

Abstract

Defining the precise promoter DNA sequence motifs where nuclear receptors and other transcription factors bind is an essential prerequisite for understanding how these proteins modulate the expression of their specific target genes. The purpose of this chapter is to provide the reader with a detailed guide with respect to the materials and the key methods required to perform this type of DNA-binding analysis. Irrespective of whether starting with purified DNA-binding proteins or somewhat crude cellular extracts, the tried-and-true procedures described here will enable one to accurately access the capacity of specific proteins to bind to DNA as well as to determine the exact sequences and DNA contact nucleotides involved. For illustrative purposes, we primarily have used the interaction of the androgen receptor with the rat probasin proximal promoter as our model system.

Impact and interest:

3 citations in Scopus
Search Google Scholar™

Citation counts are sourced monthly from Scopus and Web of Science® citation databases.

These databases contain citations from different subsets of available publications and different time periods and thus the citation count from each is usually different. Some works are not in either database and no count is displayed. Scopus includes citations from articles published in 1996 onwards, and Web of Science® generally from 1980 onwards.

Citations counts from the Google Scholar™ indexing service can be viewed at the linked Google Scholar™ search.

ID Code: 37405
Item Type: Book Chapter
Keywords: Nuclear Extracts, Methylation Interference, Androgen Receptor, Probasin Promoter, EMSA
DOI: 10.1007/978-1-60327-575-0_6
ISBN: 9781603275743
ISSN: 1064-3745
Subjects: Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > BIOCHEMISTRY AND CELL BIOLOGY (060100)
Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > GENETICS (060400) > Genome Structure and Regulation (060407)
Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > MEDICAL BIOCHEMISTRY AND METABOLOMICS (110100) > Medical Biochemistry - Nucleic Acids (110105)
Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > ONCOLOGY AND CARCINOGENESIS (111200) > Cancer Cell Biology (111201)
Divisions: Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Deposited On: 06 Oct 2010 22:40
Last Modified: 12 Oct 2016 01:30

Export: EndNote | Dublin Core | BibTeX

Repository Staff Only: item control page