Interactions between human osteoblasts and prostate cancer cells in a novel 3D in vitro model

Sieh, Shirly, Lubik, Amy, Clements, Judith A., Nelson, Colleen C., & Hutmacher, Dietmar W. (2010) Interactions between human osteoblasts and prostate cancer cells in a novel 3D in vitro model. Organogenesis, 6(3), pp. 181-188.

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Cell-cell and cell-matrix interactions play a major role in tumor morphogenesis and cancer metastasis. Therefore, it is crucial to create a model with a biomimetic microenvironment that allows such interactions to fully represent the pathophysiology of a disease for an in vitro study. This is achievable by using three-dimensional (3D) models instead of conventional two-dimensional (2D) cultures with the aid of tissue engineering technology. We are now able to better address the complex intercellular interactions underlying prostate cancer (CaP) bone metastasis through such models. In this study, we assessed the interaction of CaP cells and human osteoblasts (hOBs) within a tissue engineered bone (TEB) construct. Consistent with other in vivo studies, our findings show that intercellular and CaP cell-bone matrix interactions lead to elevated levels of matrix metalloproteinases, steroidogenic enzymes and the CaP biomarker, prostate specific antigen (PSA); all associated with CaP metastasis. Hence, it highlights the physiological relevance of this model. We believe that this model will provide new insights for understanding of the previously poorly understood molecular mechanisms of bone metastasis, which will foster further translational studies, and ultimately offer a potential tool for drug screening. © 2010 Landes Bioscience.

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32 citations in Scopus
27 citations in Web of Science®
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ID Code: 37623
Item Type: Journal Article
Refereed: Yes
Keywords: 3D in vitro model, Bone metastasis, Co-culture, Osteoblasts, Prostate cancer, Tissue engineering
DOI: 10.4161/org.6.3.12041
ISSN: 1547-6278
Divisions: Past > Schools > Cell & Molecular Biosciences
Past > QUT Faculties & Divisions > Faculty of Built Environment and Engineering
Current > Institutes > Institute of Health and Biomedical Innovation
Past > Schools > School of Engineering Systems
Copyright Owner: Copyright 2010 Landes Bioscience
Deposited On: 29 Sep 2010 23:13
Last Modified: 29 Feb 2012 14:20

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