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Differentially Expressed Protein Profile of Human Dental Pulp Cells in the Early Process of Odontoblast-like Differentiation In Vitro

Wei, Xi, Wu, Liping, Liu, Lu, Shaojun, Liu, Wei, Liu, Li, Mingtao, & Xiao, Yin (2008) Differentially Expressed Protein Profile of Human Dental Pulp Cells in the Early Process of Odontoblast-like Differentiation In Vitro. Journal of Endodontics, 34(9), pp. 1077-1084.

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    Abstract

    Dental pulp cells (DPCs) are capable of differentiating into odontoblasts that secrete reparative dentin after pulp injury. The molecular mechanisms governing reparative dentinogenesis are yet to be fully understood. Here we investigated the differential protein profile of human DPCs undergoing odontogenic induction for 7 days. Using two-dimensional differential gel electrophoresis coupled with matrix-assisted laser adsorption ionization time of flight mass spectrometry, 2 3 protein spots related to the early odontogenic differentiation were identified. These proteins included cytoskeleton proteins, nuclear proteins, cell membrane-bound molecules, proteins involved in matrix synthesis, and metabolic enzymes. The expression of four identified proteins, which were heteronuclear ribonuclear proteins C, annexin VI, collagen type VI, and matrilin-2, was confirmed by Western blot and real-time realtime polymerase chain reaction analyses. This study generated a proteome reference map during odontoblast- like differentiation of human DPCs, which will be valuable to better understand the underlying molecular mechanisms in odontoblast-like differentiation.

    Impact and interest:

    9 citations in Scopus
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    10 citations in Web of Science®

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    ID Code: 37906
    Item Type: Journal Article
    Keywords: dental pulp cells, differentiation, odontoblasts, proteome, two-dimensional differential gel electrophoresis
    DOI: 10.1016/j.joen.2008.06.014
    ISSN: 0099-2399
    Subjects: Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > BIOCHEMISTRY AND CELL BIOLOGY (060100)
    Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > CLINICAL SCIENCES (110300)
    Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > DENTISTRY (110500)
    Divisions: Past > QUT Faculties & Divisions > Faculty of Built Environment and Engineering
    Current > Institutes > Institute of Health and Biomedical Innovation
    Past > Schools > School of Engineering Systems
    Copyright Owner: Copyright 2008 Elsevier
    Deposited On: 14 Oct 2010 10:02
    Last Modified: 29 Feb 2012 23:48

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